Examination of Oral Cancer Biomarkers by Tissue Microarray Analysis
- 1 May 2008
- journal article
- research article
- Published by American Medical Association (AMA) in JAMA Otolaryngology–Head & Neck Surgery
- Vol. 134 (5), 539-46
- https://doi.org/10.1001/archotol.134.5.539
Abstract
To validate the DNA microarray results on a subset of genes that could potentially serve as biomarkers of oral squamous cell carcinoma (OSCC) by examining their expression with an alternate quantitative method and by assessing their protein levels. Based on DNA microarray data from our laboratory and data reported in the literature, we identified 6 potential biomarkers of OSCC to investigate further. We used quantitative real-time polymerase chain reaction to examine expression changes of CDH11, MMP3, SPARC, POSTN, TNC, and TGM3 in OSCC and histologically normal control tissues. We further examined validated markers at the protein level by immunohistochemical analysis of OSCC tissue microarray sections. Quantitative real-time polymerase chain reaction analysis revealed upregulation of CDH11, SPARC, POSTN, and TNC gene expression and decreased TGM3 expression in OSCC tissue compared with control tissue; MMP3 was not found to be differentially expressed. In tissue microarray immunohistochemical analyses, SPARC (secreted protein, acidic, rich in cysteine), periostin, and tenascin C exhibited increased protein expression in tumor tissue compared with control tissue, and their expression was primarily localized within tumor-associated stroma rather than tumor epithelium. Conversely, transglutaminase 3 protein expression was found only within keratinocytes in control tissue and was significantly downregulated in cancer cells. Of 6 potential gene markers of OSCC, initially identified by DNA microarray analyses, differential expression of CDH11, SPARC, POSTN, TNC, and TGM3 were validated by quantitative real-time polymerase chain reaction. Differential expression and localization of proteins encoded by SPARC, POSTN, TNC, and TGM3 were clearly shown by tissue microarray immunohistochemical analysis.Keywords
This publication has 45 references indexed in Scilit:
- Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancerBritish Journal of Cancer, 2006
- Tenascin-C patterns and splice variants in actinic keratosis and cutaneous squamous cell carcinomaBritish Journal of Dermatology, 2006
- The extracellular matrix in oral squamous cell carcinoma: Friend or foe?Head & Neck, 2006
- Genetic expression profiles and biologic pathway alterations in head and neck squamous cell carcinomaCancer, 2005
- Novel markers for poor prognosis in head and neck cancerInternational Journal of Cancer, 2004
- Gene expression signature predicts lymphatic metastasis in squamous cell carcinoma of the oral cavityOncogene, 2004
- Gene expression profiling of oral squamous cell carcinoma using laser microdissection and cDNA microarrayMedical Molecular Morphology, 2004
- Distinct pattern of expression of differentiation and growth-related genes in squamous cell carcinomas of the head and neck revealed by the use of laser capture microdissection and cDNA arraysOncogene, 2000
- Characteristic Distribution Patterns of Tenascin in Laryngeal and Hypopharyngeal CancersThe Laryngoscope, 2000
- THE DISTRIBUTION OF SECONDARY GROWTHS IN CANCER OF THE BREAST.The Lancet, 1889