Novel Plant Immune-Priming Compounds Identified via High-Throughput Chemical Screening Target Salicylic Acid Glucosyltransferases in Arabidopsis
Open Access
- 1 September 2012
- journal article
- Published by Oxford University Press (OUP) in THE PLANT CELL ONLINE
- Vol. 24 (9), 3795-3804
- https://doi.org/10.1105/tpc.112.098343
Abstract
Plant activators are compounds, such as analogs of the defense hormone salicylic acid (SA), that protect plants from pathogens by activating the plant immune system. Although some plant activators have been widely used in agriculture, the molecular mechanisms of immune induction are largely unknown. Using a newly established high-throughput screening procedure that screens for compounds that specifically potentiate pathogen-activated cell death in Arabidopsis thaliana cultured suspension cells, we identified five compounds that prime the immune response. These compounds enhanced disease resistance against pathogenic Pseudomonas bacteria in Arabidopsis plants. Pretreatments increased the accumulation of endogenous SA, but reduced its metabolite, SA-O-β-d-glucoside. Inducing compounds inhibited two SA glucosyltransferases (SAGTs) in vitro. Double knockout plants that lack both SAGTs consistently exhibited enhanced disease resistance. Our results demonstrate that manipulation of the active free SA pool via SA-inactivating enzymes can be a useful strategy for fortifying plant disease resistance and may identify useful crop protectants.Keywords
This publication has 37 references indexed in Scilit:
- The Arabidopsis Glucosyltransferase UGT76B1 Conjugates Isoleucic Acid and Modulates Plant Defense and SenescenceTHE PLANT CELL ONLINE, 2011
- Salicylic Acid Biosynthesis and MetabolismThe Arabidopsis Book, 2011
- Salicylic Acid, a Multifaceted Hormone to Combat DiseaseAnnual Review of Phytopathology, 2009
- The Synthetic Elicitor 3,5-Dichloroanthranilic Acid InducesNPR1-Dependent andNPR1-Independent Mechanisms of Disease Resistance in ArabidopsisPlant Physiology, 2009
- A Kinetic Analysis of Regiospecific Glucosylation by Two Glycosyltransferases of Arabidopsis thaliana: DOMAIN SWAPPING TO INTRODUCE NEW ACTIVITIESOnline Journal of Public Health Informatics, 2008
- RIN4 Interacts with Pseudomonas syringae Type III Effector Molecules and Is Required for RPM1-Mediated Resistance in ArabidopsisCell, 2002
- Plant pathogens and integrated defence responses to infectionNature, 2001
- THE OXIDATIVE BURST IN PLANT DISEASE RESISTANCEAnnual Review of Plant Physiology and Plant Molecular Biology, 1997
- Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance.THE PLANT CELL ONLINE, 1991
- The Characteristics of Probenazole (Oryzemate®) for the Control of Rice BlastJournal of Pesticide Science, 1977