Identification of cis -regulatory sequences that activate transcription in the suspensor of plant embryos
- 3 March 2009
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 106 (9), 3627-3632
- https://doi.org/10.1073/pnas.0813276106
Abstract
Little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. We analyzed the upstream region of the scarlet runner bean (Phaseolus coccineus) G564 gene to understand how genes are activated specifically within the suspensor during early embryo development. Previously, we showed that the G564 upstream region has a block of tandem repeats, which contain a conserved 10-bp motif (GAAAAGC/TGAA), and that deletion of these repeats results in a loss of suspensor transcription. Here, we use gain-of-function (GOF) experiments with transgenic globular-stage tobacco embryos to show that only 1 of the 5 tandem repeats is required to drive suspensor-specific transcription. Fine-scale deletion and scanning mutagenesis experiments with 1 tandem repeat uncovered a 54-bp region that contains all of the sequences required to activate transcription in the suspensor, including the 10-bp motif (GAAAAGCGAA) and a similar 10-bp-like motif (GAAAAACGAA). Site-directed mutagenesis and GOF experiments indicated that both the 10-bp and 10-bp-like motifs are necessary, but not sufficient to activate transcription in the suspensor, and that a sequence (TTGGT) between the 10-bp and the 10-bp-like motifs is also necessary for suspensor transcription. Together, these data identify sequences that are required to activate transcription in the suspensor of a plant embryo after fertilization.Keywords
This publication has 27 references indexed in Scilit:
- The MYB98 subcircuit of the synergid gene regulatory network includes genes directly and indirectly regulated by MYB98The Plant Journal, 2008
- The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuronGenes & Development, 2007
- Using Genomics to Study Legume Seed DevelopmentPlant Physiology, 2007
- Transcriptional regulation of epidermal cell fate in theArabidopsisembryoDevelopment, 2007
- Identification of Genes that are Up- or Down-regulated in the Apical or Basal Cell of Maize Two-celled Embryos and Monitoring their Expression During Zygote Development by a Cell Manipulation- and PCR-based ApproachPlant and Cell Physiology, 2005
- WebLogo: A Sequence Logo Generator: Figure 1Genome Research, 2004
- Plant Embryogenesis: Zygote to SeedScience, 1994
- Embryogenesis in Angiosperms: Development of the Suspensor.Plant Cell, 1993
- Embryogenesis in Angiosperms: Development of the SuspensorPlant Cell, 1993
- Binding sites for two novel phosphoproteins, 3AF5 and 3AF3, are required for rbcS-3A expression.Plant Cell, 1992