PURIFIED PROTEIN DERIVATIVE OF TUBERCULIN INDUCES IMMUNOGLOBULIN PRODUCTION IN NORMAL MOUSE SPLEEN CELLS
Open Access
- 1 January 1973
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 137 (1), 127-139
- https://doi.org/10.1084/jem.137.1.127
Abstract
Purified protein derivative (PPD) tuberculin induced immunoglobulin production in cultures of nonimmune mouse spleen cells, as measured by plaque-forming cells (PFC) against sheep erythrocytes (SRBC), horse erythrocytes, and 4-hydroxy-3,5-dinitrophenacetyl-SRBC. The increase started between 15 and 20 h of culture and reached a peak at 48–72 h. Higher PPD concentrations resulted in earlier peak responses than low concentrations. The Ig produced was mainly 19S and of very low avidity. The response elicited by PPD was of the same type as that caused by lipopolysaccharide of bacterial origin. Mitomycin treatment of cells before culture did not change the numbers of PFC/106 recovered cells but the cell recovery was considerably lower.Keywords
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