The cytoplasmic carboxy terminus of M13 procoat is required for the membrane insertion of its central domain
- 1 July 1986
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 322 (6077), 335-339
- https://doi.org/10.1038/322335a0
Abstract
The M13 coat protein spans the Escherichia coli plasma membrane with its amino-terminus facing the periplasm. It is made as a precursor--the procoat--with a typical leader peptide. Mutations which destroy the basic character of the carboxy-terminal domain of procoat, a domain which is oriented towards the cytoplasm, block membrane assembly, while insertion of three lysyl residues near the carboxy terminus partially restores assembly. Thus the information specifying membrane insertion of M13 procoat protein is found in its mature region as well as the leader and is not simply decoded in an amino to carboxy direction.This publication has 22 references indexed in Scilit:
- Multiple Mechanisms of Protein Insertion into and Across MembranesScience, 1985
- A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptidesJournal of Molecular Biology, 1983
- Patterns of Amino Acids near Signal‐Sequence Cleavage SitesJBIC Journal of Biological Inorganic Chemistry, 1983
- Diverse effects of mutations in the signal sequence on the secretion of β-lactamase in Salmonella typhimuriumCell, 1982
- Mechanism of Incorporation of Cell Envelope Proteins in Escherichia ColiAnnual Review of Microbiology, 1982
- Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertaseCell, 1982
- Role of the mature protein sequence of maltose-binding protein in its secretion across the E. coli cytoplasmic membraneCell, 1981
- Secretion of beta-lactamase requires the carboxy end of the proteinCell, 1980
- Intracellular protein topogenesisProceedings of the National Academy of Sciences of the United States of America, 1980
- A Possible Precursor of Immunoglobulin Light ChainsNature New Biology, 1972