Genetic diversity among Plasmodium falciparum field isolates in Pakistan measured with PCR genotyping of the merozoite surface protein 1 and 2
Top Cited Papers
Open Access
- 1 January 2010
- journal article
- Published by Springer Science and Business Media LLC in Malaria Journal
- Vol. 9 (1), 1
- https://doi.org/10.1186/1475-2875-9-1
Abstract
Background: The genetic diversity of Plasmodium falciparum has been extensively studied in various parts of the world. However, limited data are available from Pakistan. This study aimed to establish molecular characterization of P. falciparum field isolates in Pakistan measured with two highly polymorphic genetic markers, i.e. the merozoite surface protein 1 (msp-1)and 2 (msp-2). Methods: Between October 2005 and October 2007, 244 blood samples from patients with symptomatic blood-slide confirmed P. falciparum mono-infections attending the Aga Khan University Hospital, Karachi, or its collection units located in Sindh and Baluchistan provinces, Pakistan were collected. The genetic diversity of P. falciparum was analysed by length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) targeting block 2 of msp-1 and block 3 of msp-2, including their respective allelic families KI, MAD 20, RO33, and FC27, 3D7/IC. Results: A total of 238/244 (98%) patients had a positive PCR outcome in at least one genetic marker; the remaining six were excluded from analysis. A majority of patients had monoclonal infections. Only 56/231 (24%) and 51/236 (22%) carried multiple P. falciparum genotypes in msp-1 and msp-2, respectively. The estimated total number of genotypes was 25 msp-1 (12 KI; 8 MAD20; 5 RO33) and 33 msp-2 (14 FC27; 19 3D7/IC). Conclusions: This is the first report on molecular characterization of P. falciparum field isolates in Pakistan with regards to multiplicity of infection. The genetic diversity and allelic distribution found in this study is similar to previous reports from India and Southeast Asian countries with low malaria endemicity.This publication has 27 references indexed in Scilit:
- Genetic structure of Plasmodium falciparum field isolates in eastern and north-eastern IndiaMalaria Journal, 2007
- Influence of Consecutive?Day Blood Sampling on Polymerase Chain Reaction–Adjusted Parasitological Cure Rates in an Antimalarial?Drug Trial Conducted in TanzaniaThe Journal of Infectious Diseases, 2007
- Genetic diversity in merozoite surface protein (MSP)-1 and MSP-2 genes of Plasmodium falciparum in a major endemic region of IranThe Korean Journal of Parasitology, 2007
- Multiple genotypes of the merozoite surface proteins 1 and 2 in Plasmodium falciparum infections in a hypoendemic area in IranTropical Medicine & International Health, 2005
- Genotyping of Plasmodium falciparum infections by PCR: a comparative multicentre studyTransactions of the Royal Society of Tropical Medicine and Hygiene, 2001
- Resistance of Plasmodium falciparum malaria to chloroquine is widespread in eastern AfghanistanPathogens and Global Health, 2001
- Complexity ofPlasmodium falciparumInfections Is Consistent over Time and Protects against Clinical Disease in Tanzanian ChildrenThe Journal of Infectious Diseases, 1999
- ACP Broadsheet no 148. July 1996. Laboratory diagnosis of malaria.Journal of Clinical Pathology, 1996
- Gene flow and cross-mating in Plasmodium falciparum in households in a Tanzanian villageParasitology, 1995
- The major merozoite surface protein as a malaria vaccine targetParasitology Today, 1993