Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response
- 1 May 2012
- journal article
- Published by Taylor & Francis Ltd in Plant Signaling & Behavior
- Vol. 7 (5), 547-550
- https://doi.org/10.4161/psb.19803
Abstract
The heat stress (HS) response in eukaryotes is mainly regulated by heat shock factors (HSFs). Genetic disruption of the master HSF gene leads to dramatically reduced HS response and thermotolerance in several model organisms. However, it is not clear whether organisms devoid of the master regulator can still acclimate to heat. Previously, we showed that Arabidopsis HsfA1a, HsfA1b, and HsfA1d act as master regulators in the HS response. In this study, we examined the heat acclimation capacity of the Arabidopsis quadruple and triple T-DNA knockout mutants of HsfA1a, HsfA1b, HsfA1d, and HsfA1e. Our data showed that in the absence of the master regulators, a minimal but significant level of acquired thermotolerance could be attained in the Arabidopsis mutants after acclimation. The optimum acclimation temperature for the HsfA1 quadruple mutant was lower than that for the wild type plants, suggesting that plant cells have two HS-sensing mechanisms that can be distinguished genetically. The acquired thermotolerance of the quadruple mutant was likely due to the induction of a small number of HsfA1-independent HS response genes regulated by other transcription factors. Here, we discuss the possible candidates and propose a working model of the transcription network of the HS response by including the HsfA1-dependent and -independent pathways.Keywords
This publication has 38 references indexed in Scilit:
- The HSF-like Transcription Factor TBF1 Is a Major Molecular Switch for Plant Growth-to-Defense TransitionCurrent Biology, 2012
- Two different heat shock transcription factors regulate immediate early expression of stress genes in ArabidopsisMolecular Genetics and Genomics, 2003
- Regulation of Molecular Chaperone Gene Transcription Involves the Serine Phosphorylation, 14-3-3ε Binding, and Cytoplasmic Sequestration of Heat Shock Factor 1Molecular and Cellular Biology, 2003
- Redox regulation of mammalian heat shock factor 1 is essential for Hsp gene activation and protection from stressGenes & Development, 2003
- In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomatoGenes & Development, 2002
- A rice spotted leaf gene, Spl7 , encodes a heat stress transcription factor proteinProceedings of the National Academy of Sciences of the United States of America, 2002
- Targeted disruption of hsf1 leads to lack of thermotolerance and defines tissue‐specific regulation for stress‐inducible Hsp molecular chaperonesJournal of Cellular Biochemistry, 2002
- Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1The EMBO Journal, 2001
- Heat Shock Protein 101 Plays a Crucial Role in Thermotolerance in ArabidopsisPlant Cell, 2000
- THE HEAT-SHOCK RESPONSEAnnual Review of Biochemistry, 1986