Multiplex Polymerase Chain Reaction for Identification and Differentiation of Streptococcus equi subsp. zooepidemicus and Streptococcus equi subsp. equi
- 6 December 2004
- journal article
- research article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 51 (10), 455-458
- https://doi.org/10.1111/j.1439-0450.2004.00799.x
Abstract
The closely related streptococcal species Streptococcus equi subsp. zooepidemicus and S. equi subsp. equi were identified by polymerase chain reaction using oligonucleotide primers designed according to species-specific parts of the superoxide dismutase A encoding gene sodA. A further differentiation of both subspecies could be performed by amplification of the genes seeH and seeI encoding the exotoxins SeeH and SeeI, respectively, which could be detected for S. equi subsp. equi but not for S. equi subsp. zooepidemicus. A further simplification of the identification and differentiation of both subspecies was conducted by sodA-seeI multiplex polymerase chain reaction.Keywords
This publication has 39 references indexed in Scilit:
- Identification of Isolates of Streptococcus canis Infecting HumansJournal of Clinical Microbiology, 2001
- Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberisJournal of Clinical Microbiology, 2001
- Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp.zooepidemicusResearch in Veterinary Science, 2000
- Amplification of 16S ribosomal RNA gene sequences for the identification of streptococci of Lancefield group BResearch in Veterinary Science, 1999
- Species identification of Streptococcus porcinus by restriction fragment length polymorphism analysis of 16S ribosomal DNAResearch in Veterinary Science, 1998
- Determination of 16S ribosomal RNA gene copy number inStreptococcus uberis, S. agalactiae, S. dysgalactiaeandS. parauberisFEMS Immunology & Medical Microbiology, 1995
- Determination of 16S rRNA Sequences of Streptococcus mitis and Streptococcus gordonii and Phylogenetic Relationships among Members of the Genus StreptococcusInternational Journal of Systematic and Evolutionary Microbiology, 1995
- Intrageneric Structure of Streptococcus Based on Comparative Analysis of Small-Subunit rRNA SequencesInternational Journal of Systematic and Evolutionary Microbiology, 1991
- NEPHRITIS CAUSED BY STREPTOCOCCUS ZOOEPIDEMICUS (LANCEFIELD GROUP C)The Lancet, 1983
- A new nephritogenic streptococcusEpidemiology and Infection, 1969