Mitochondrial protein import: Nucleoside triphosphates are involved in conferring import-competence to precursors
- 19 June 1987
- journal article
- Published by Elsevier BV in Cell
- Vol. 49 (6), 815-823
- https://doi.org/10.1016/0092-8674(87)90619-2
Abstract
The role of nucleoside triphosphates (NTPs) in mitochondrial protein import was investigated with the precursors of N. crassa ADP/ATP carrier, F1-ATPase subunit beta, F0-ATPase subunit 9, and fusion proteins between subunit 9 and mouse dihydrofolate reductase. NTPs were necessary for the initial interaction of precursors with the mitochondria and for the completion of translocation of precursors from the mitochondrial surface into the mitochondria. Higher levels of NTPs were required for the latter reactions as compared with the early stages of import. Import of precursors having identical presequences but different mature protein parts required different levels of NTPs. The sensitivity of precursors in reticulocyte lysate to proteases was decreased by removal of NTPs and increased by their readdition. We suggest that the hydrolysis of NTPs is involved in modulating the folding state of precursors in the cytosol, thereby conferring import competence.Keywords
This publication has 38 references indexed in Scilit:
- The transmembrane arrangement of the ADP/ATP carrier as elucidated by the lysine reagent pyridoxal 5‐phosphateEuropean Journal of Biochemistry, 1986
- Binding of a specific ligand inhibits import of a purified precursor protein into mitochondriaNature, 1986
- The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal.The Journal of cell biology, 1986
- The precursor to ornithine carbamyl transferase is transported to mitochondria as a 5S complex containing an import factorBiochemical and Biophysical Research Communications, 1985
- Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNADNA, 1985
- RNA Required for Import of Precursor Proteins into MitochondriaScience, 1984
- Surface labeling of membrane‐bound ADP/ATP carrier by pyridoxal phosphateFEBS Letters, 1982
- Processing of pre-ornithine transcarbamylase requires a zinc-dependent protease localized to the mitochondrial matrixBiochemical and Biophysical Research Communications, 1982
- Fluorographic detection of radioactivity in polyacrylamide gols with the water-soluble fluor, sodium salicylateAnalytical Biochemistry, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976