High-performance liquid chromatographic purification of peptide hormones: Ovine hypothalamic amunine (corticotropin releasing factor)
- 6 December 1982
- journal article
- Published by Elsevier BV in Analytical Biochemistry
- Vol. 127 (2), 258-266
- https://doi.org/10.1016/0003-2697(82)90170-1
Abstract
Amunine, or corticotropin releasing factor (CRF), a 41-peptide amide, has been isolated from ovine hypothalami. Tissues were extracted, defatted, and partitioned prior to being sized by gel permeation. The active zone followed by a sensitive in vitro assay (CRF-mediated corticotropin release from pituitary cells in monolayer cultures quantitated by a specific corticotropin radioim munoassay) could be purified by reverse-phase high-performance liquid chromatography. Column characteristics (i.e., properties of the derivatized silicas including pore size), selection of mobile phases, and temperature effects are discussed.Keywords
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