Development of molecular markers linked to the ‘Fiesta’ linkage group 7 major QTL for fire blight resistance and their application for marker-assisted selection
- 1 June 2007
- journal article
- Published by Canadian Science Publishing in Genome
- Vol. 50 (6), 568-577
- https://doi.org/10.1139/g07-033
Abstract
A fire blight resistance QTL explaining 34.3%–46.6% of the phenotypic variation was recently identified on linkage group 7 of apple cultivar ‘Fiesta’ (F7). However, markers flanking this QTL were AFLP and RAPD markers unsuitable for marker-assisted selection (MAS). Two RAPD markers bracketing the QTL have been transformed into SCAR (sequence-characterized amplified region) markers, and an SSR marker specific for the region was developed. Pedigree analysis of ‘Fiesta’ with these markers enabled tracking of the F7 QTL allele back to ‘Cox’s Orange Pippin’. Stability of the effect of this QTL allele in different backgrounds was analyzed by inoculating progeny plants of a cross between ‘Milwa’, a susceptible cultivar, and ‘1217’, a moderately resistant cultivar, and a set of cultivars that carry or lack the allele conferring increased fire blight resistance. Progenies and cultivars that carried both markers were significantly more resistant than those that did not carry both markers, indicating high stability of the F7 QTL allele in different backgrounds. This stability and the availability of reproducible markers bracketing the QTL make this locus promising for use in MAS.Keywords
This publication has 25 references indexed in Scilit:
- Venturia inaequalisResistance in AppleCritical Reviews in Plant Sciences, 2006
- Mapping of the apple scab-resistance gene VbGenome, 2006
- Microsatellite markers spanning the apple (Malus x domestica Borkh.) genomeTree Genetics & Genomes, 2006
- QTL mapping of fire blight resistance in appleMolecular Breeding, 2006
- Identifying QTLs for fire-blight resistance via a European pear (Pyrus communis L.) genetic linkage mapMolecular Breeding, 2004
- Confirmation of QTL Associated with Common Bacterial Blight Resistance in Four Different Genetic Backgrounds in Common BeanCrop Science, 1999
- DNA markers linked to Malus floribunda 821 scab resistancePlant Molecular Biology, 1994
- Quantitative trait loci for plant height in four maize populations and their associations with qualitative genetic lociTheoretical and Applied Genetics, 1991
- Differential Host × Pathogen Interactions Among Cultivars of Apple and Strains ofErwinia amylovoraPhytopathology®, 1984
- Procedures for Detecting Outlying Observations in SamplesTechnometrics, 1969