A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity
- 1 June 2007
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 99 (1), 129-135
- https://doi.org/10.1002/bit.21521
Abstract
A novel three stages continuous fermentation process for the bioproduction of succinic acid at high concentration, productivity and yield using A. succiniciproducens was developed. This process combined an integrated membrane‐bioreactor‐electrodialysis system. An energetic characterization of A. succiniciproducens during anaerobic cultured in a cell recycle bioreactor was done first. The very low value of YATP obtained suggests that an ATP dependent mechanism of succinate export is present in A. succiniciproducens. Under the best culture conditions, biomass concentration and succinate volumetric productivity reach values of 42 g/L and 14.8 g/L·h. These values are respectively 28 and 20 times higher compared to batch cultures done in our laboratory. To limit end‐products inhibition on growth, a mono‐polar electrodialysis pilot was secondly coupled to the cell recycle bioreactor. This system allowed to continuously remove succinate and acetate from the permeate and recycle an organic acids depleted solution in the reactor. The integrated membrane‐bioreactor‐electrodialysis process produced a five times concentrated succinate solution (83 g/L) compared to the cell recycle reactor system, at a high average succinate yield of 1.35 mol/mol and a slightly lower volumetric productivity of 10.4 g/L·h. The process combined maximal production yield to high productivity and titer and could be economically viable for the development of a biological route for succinic acid production. Biotechnol. Bioeng. 2008;99: 129–135.Keywords
This publication has 21 references indexed in Scilit:
- High production of acetone–butanol–ethanol with high cell density culture by cell-recycling and bleedingJournal of Biotechnology, 2005
- Fed‐batch culture of a metabolically engineered Escherichia coli strain designed for high‐level succinate production and yield under aerobic conditionsBiotechnology & Bioengineering, 2005
- Genetic reconstruction of the aerobic central metabolism inEscherichia coli for the absolute aerobic production of succinateBiotechnology & Bioengineering, 2004
- Microbial export of lactic and 3-hydroxypropanoic acid: implications for industrial fermentation processesMetabolic Engineering, 2004
- Fermentative Production of Chemicals That Can Be Used for Polymer SynthesisMacromolecular Bioscience, 2004
- Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coliApplied and Environmental Microbiology, 2004
- Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coliApplied and Environmental Microbiology, 2002
- The fermentation pathways ofEscherichia coliFEMS Microbiology Letters, 1989
- Kinetics of ethanol fermentations in membrane cell recycle fermentorsBiotechnology & Bioengineering, 1987
- Acetonobutylic fermentation: Improvement of performances by coupling continuous fermentation and ultrafiltrationBiotechnology & Bioengineering, 1986