Aedes (Stegomyia) albopictus (Skuse): A Potential Vector of Zika Virus in Singapore

Abstract

Zika virus (ZIKV) is a little known arbovirus until it caused a major outbreak in the Pacific Island of Yap in 2007. Although the virus has a wide geographic distribution, most of the known vectors are sylvatic Aedes mosquitoes from Africa where the virus was first isolated. Presently, Ae. aegypti is the only known vector to transmit the virus outside the African continent, though Ae. albopictus has long been a suspected vector. Currently, Ae. albopictus has been shown capable of transmitting more than 20 arboviruses and its notoriety as an important vector came to light during the recent chikungunya pandemic. The vulnerability of Singapore to emerging infectious arboviruses has stimulated our interest to determine the competence of local Ae. albopictus to transmit ZIKV. To determine the competence of Ae. albopictus to ZIKV, we orally infected local mosquito strains to a Ugandan strain virus. Fully engorged mosquitoes were maintained in an environmental chamber set at 29°C and 80–85%RH. Twelve mosquitoes were then sampled daily from day one to seven and on day 10 and 14 post infection (pi). Zika virus titre in the midgut and salivary glands of each mosquito were determined using tissue culture infectious dose₅₀ assay, while transmissibility of the virus was determined by detecting viral antigen in the mosquito saliva by qRT-PCR. High dissemination and transmission rate of ZIKV were observed. By day 7-pi, all mosquitoes have disseminated infection and 73% of these mosquitoes have ZIKV in their saliva. By day 10-pi, all mosquitoes were potentially infectious. The study highlighted the potential of Ae. albopictus to transmit ZIKV and the possibility that the virus could be established locally. Nonetheless, the threat of ZIKV can be mitigated by existing dengue and chikungunya control program being implemented in Singapore. The prominence of Ae. albopictus as a major vector came to light during the global pandemic of chikungunya. The continual global expansion of Ae. albopictus coupled with data from competence studies that show its ability to transmit more than 20 arboviruses has fuelled growing concerns that this mosquito may alter the epidemiology of arbovirus worldwide and increase the risks of humans to mosquito-borne diseases. This has stimulated our interest to determine the extent of pathogens Ae. albopictus can transmit, especially arboviruses with high epidemic potential. The current study showed that local Ae. albopictus are susceptible to ZIKV. By day 7 pi. all mosquitoes that were orally infected were found to have disseminated infections and 73% have ZIKV in their saliva. This study highlighted the potential of Ae. albopictus to transmit ZIKV. The local transmission of ZIKV in Singapore is plausible due to the presence of susceptible vectors, an immunologically naive and vulnerable human population, and the travel hub status of Singapore. Nonetheless, the threat of ZIKV can be mitigated by existing dengue and chikungunya control program being implemented in Singapore.