Sequence analysis of the gtfB gene from Streptococcus mutans
Open Access
- 1 September 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (9), 4263-4270
- https://doi.org/10.1128/jb.169.9.4263-4270.1987
Abstract
The nucleotide sequence of the gtfB gene from Streptococcus mutans GS-5, coding for glucosyltransferase I activity, was determined. The gene codes for a strongly hydrophilic protein with a molecular size of 165,800 daltons. The deduced amino acid sequence revealed a typical gram-positive bacterial signal sequence at the NH2 terminus of the protein and 3.5 direct repeating units (each containing 65 amino acids) at the COOH terminus. Nucleotide sequencing of the region immediately downstream from the gtfB gene revealed the presence of a putative gene coding for an extracellular protein. This open reading frame is partially homologous to the gtfB gene.Keywords
This publication has 18 references indexed in Scilit:
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Genetic Analysis of Streptococcus mutans VirulencePublished by Springer Science and Business Media LLC ,1985
- Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingGene, 1984
- Three kinds of extracellular glucosyltransferases fromStreptococcus mutans6715 (serotypeg)FEBS Letters, 1983
- Patterns of Amino Acids near Signal‐Sequence Cleavage SitesEuropean Journal of Biochemistry, 1983
- Purification and characterization of basic glucosyltransferase from Streptococcus mutans serotype cBiochimica et Biophysica Acta (BBA) - General Subjects, 1982
- A mechanism of protein localization: the signal hypothesis and bacteria.The Journal of cell biology, 1980
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979
- Dental CariesAnnual Review of Medicine, 1975
- Regulation of Escherichia coli phosphofructokinase in situBiochemical and Biophysical Research Communications, 1973