Collaborative study of the MPN, Anderson–Baird-Parker direct plating, and hydrophobic grid-membrane filter methods for the enumeration of Escherichia coli biotype I in foods
- 1 October 1983
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 29 (10), 1247-1252
- https://doi.org/10.1139/m83-194
Abstract
Five Health Protection Branch laboratories compared two membrane filter methods (the Anderson–Baird-Parker direct plating, and a hydrophobic grid-membrane filter method) against the most probable number procedure (MPN) for enumerating Escherichia coli biotype I in foods. Results were available in 24 h by both membrane filter methods, compared with 10–14 days by the MPN procedure. For ground beef, Parmesan cheese, and cut green beans, the hydrophobic grid method generally gave the highest recovery, although the two membrane filter methods were not significantly different. Both these methods gave significantly higher recoveries than the MPN procedure, and for most foods, either method would be preferable. Further work is required before either membrane filter method can be recommended for bean and alfalfa sprouts, which may contain very high levels of Klebsiella spp.This publication has 5 references indexed in Scilit:
- Colony counting on hydrophobic grid-membrane filtersCanadian Journal of Microbiology, 1983
- Enumeration of Indicator Organisms in Foods Using the Automated Hydrophobic Grid-Membrane Filter TechniqueJournal of Food Protection, 1982
- Filtering out food debris before microbiological analysisApplied and Environmental Microbiology, 1981
- Membrane filtration of dairy products for microbiological analysisApplied and Environmental Microbiology, 1980
- Improved detection of coliforms and Escherichia coli in foods by a membrane filter methodApplied and Environmental Microbiology, 1979