Calcium deposition in osteoarthritic meniscus and meniscal cell culture
Open Access
- 30 March 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Arthritis Research & Therapy
- Vol. 12 (2), R56
- https://doi.org/10.1186/ar2968
Abstract
Calcium crystals exist in the knee joint fluid of up to 65% of osteoarthritis (OA) patients and the presence of these calcium crystals correlates with the radiographic evidence of hyaline cartilaginous degeneration. This study sought to examine calcium deposition in OA meniscus and to investigate OA meniscal cell-mediated calcium deposition. The hypothesis was that OA meniscal cells may play a role in pathological meniscal calcification. Studies were approved by our human subjects Institutional Review Board. Menisci were collected during joint replacement surgeries for OA patients and during limb amputation surgeries for osteosarcoma patients. Calcium deposits in menisci were examined by alizarin red staining. Expression of genes involved in biomineralization in OA meniscal cells was examined by microarray and real-time RT-PCR. Cell-mediated calcium deposition in monolayer culture of meniscal cells was examined using an ATP-induced 45calcium deposition assay. Calcium depositions were detected in OA menisci but not in normal menisci. The expression of several genes involved in biomineralization including ENPP1 and ANKH was upregulated in OA meniscal cells. Consistently, ATP-induced calcium deposition in the monolayer culture of OA meniscal cells was much higher than that in the monolayer culture of control meniscal cells. Calcium deposition is common in OA menisci. OA meniscal cells calcify more readily than normal meniscal cells. Pathological meniscal calcification, which may alter the biomechanical properties of the knee meniscus, is potentially an important contributory factor to OA.Keywords
This publication has 50 references indexed in Scilit:
- Analysis of meniscal degeneration and meniscal gene expressionBMC Musculoskeletal Disorders, 2010
- Telomerase Transduced Osteoarthritis Fibroblast-Like Synoviocytes Display a Distinct Gene Expression ProfileThe Journal of Rheumatology, 2009
- Incidental Meniscal Findings on Knee MRI in Middle-Aged and Elderly PersonsNew England Journal of Medicine, 2008
- Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan productionBMC Biotechnology, 2008
- Characterization of articular calcium-containing crystals by synchrotron FTIROsteoarthritis and Cartilage, 2008
- OsteoarthritisJournal of Cellular Physiology, 2007
- Osteopontin promotes pathologic mineralization in articular cartilageMatrix Biology, 2007
- Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signallingArthritis Research & Therapy, 2007
- Basic calcium phosphate crystal-induced Egr-1 expression stimulates mitogenesis in human fibroblastsBiochemical and Biophysical Research Communications, 2005
- Basic calcium phosphate crystals cause coordinate induction and secretion of collagenase and stromelysinJournal of Cellular Physiology, 1992