In view of the underground moving target localization question, a solution has been proposed which is based on the integration of RFID and GIS. This paper presents relevant concepts of the RFID and GIS; analyzes the working principle, RFID reader positioning error and its installation methods as well, which are on the basis of the RFID and GIS moving target positioning system. In the paper, each of the moving targets carries an RFID tag which has the property information of the moving target. The distances from every RFID readers to their laneway entry have been stored in the database in order that the underground reader calculates and locates the position of moving target. Therefore, the system can realize the localization and track on underground moving targets, and thus can increase aid effectiveness by locating the affected staffs quickly and accurately. Moreover, the system is convenient for maintenance and ease to manage.