Genetic characterization of a novelPhomopsissp., a putative biocontrol agent forCarthamus lanatus
- 20 January 2010
- journal article
- Published by Taylor & Francis Ltd in Mycologia
- Vol. 102 (1), 54-61
- https://doi.org/10.3852/08-198
Abstract
A large number of isolates of Phomopsis sp. have been collected from the weed Carthamus lanatus (saffron thistle) in Australia, and their potential as biological control agents for weeds of the Asteraceae has been demonstrated. An analysis of their genetic diversity and a multigene phylogenetic analysis were undertaken to ascertain whether these isolates were distinct from other species of Phomopsis that commonly attack crop species in Australia. Minimal variation was found between the Phomopsis spp. isolated from saffron thistle, except two isolates that appeared to share identity with Diaporthe helianthii and P. viticola. Analysis of the selected isolates from saffron thistle with the nucleotide sequence of the partial ITS and tef1-α regions demonstrated that the sequences were distinct from all other species of Phomopsis so far described from crops in Australia. These findings provide strong support for the recognition of these isolates as a separate species of Phomopsis. The implications of these findings are discussed in relation to biological control of saffron thistle.Keywords
This publication has 30 references indexed in Scilit:
- Real-time PCR quantification ofColletotrichum coccodesDNA in soils from bioherbicide field-release assays, with normalization for PCR inhibitionCanadian Journal of Plant Pathology, 2006
- Molecular identification of fungi isolated from stem tissue of Upland cotton (Gossypium hirsutum)Australian Journal of Botany, 2005
- Influence of crop management on sunflower stem canker (Diaporthe helianthi)Agronomy for Sustainable Development, 2003
- An investigation of genetic variation in Carthamus lanatus in New South Wales, Australia, using intersimple sequence repeats (ISSR) analysisWeed Research, 2003
- Risk Analysis of Sclerotinia sclerotiorum for Biological Control of Cirsium arvense in Pasture: Sclerotium SurvivalBiocontrol Science and Technology, 2000
- Characterization of Bifidobacterium Strains Using Box PrimersAnaerobe, 2000
- PATTERNS OF DESCENT IN CLONAL LINEAGES AND THEIR MULTILOCUS FINGERPRINTS ARE RESOLVED WITH COMBINED GENE GENEALOGIESEvolution, 1999
- DISEASE NOTES OR NEW RECORDS: Phomopsis spp. associated with die-back of saffron thistle (Carthamuslanatus)Australasian Plant Pathology, 1996
- Constraints in the Development of BioherbicidesWeed Technology, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994