Cadmium Chloride and Cadmium Metallothionein-Induced Pulmonary Injury and Recruitment of Polymorphonuclear Leukocytes
- 1 January 1994
- journal article
- Published by Taylor & Francis Ltd in Experimental Lung Research
- Vol. 20 (6), 517-537
- https://doi.org/10.3109/01902149409031735
Abstract
Pulmonary exposure of rats to either cadmium chloride (CdCl2) or cadmium metallothionein (CdMT) was previously reported to induce an influx of polymorphonuclear leukocytes (PMNs) to the airways, but only CdCl2 caused a significant increase in lung permeability, indicative of damage to the pulmonary epithelium. The purpose of this study was to investigate mechanisms of PMN recruitment following exposure to these forms of cadmium. Fischer 344 rats were intratracheally instilled with 10 μg cadmium in the form of CdCl2 or CdMT, and the time course of pulmonary inflammation and PMN migration activity was determined. PMN numbers, permeability, and PMN migration activity of lung lavage supernatant peaked 1 to 2 days after CdCl2 exposure. PMN migration activity was not detected 5 h after CdMT exposure, despite a peak of PMN numbers 10 h after exposure, but was increased by 1 day when permeability had increased to a small but significant degree. Elastase-modified forms of α-1-proteinase inhibitor (α1,PI), with molecular weights of 80 and 51 kd, have been reported to be highly chemotactic for PMNs. Antiserum to α1PI significantly inhibited PMN migration activity detected in supernatants 1 day after exposure to either CdCl2 or CdMT. The results suggest that both CdCl2 and CdMT induce the formation of high molecular weight modified forms of α1 PI in the airways; these factors may traverse damaged epithelium to recruit PMNs from the vasculature. Additional small or lipophilic factors, undetectable by the methods of this study, may be responsible for the early influx of PMNs following CdMT exposure in the absence of increased epithelial permeability.Keywords
This publication has 30 references indexed in Scilit:
- Lung Clearance Mechanisms of Soluble and Insoluble Cadmium CompoundsPublished by Springer Science and Business Media LLC ,1990
- A comparative study of the effects of inhaled cadmium chloride and cadmium oxide: Pulmonary responseJournal of Toxicology and Environmental Health, 1987
- Detection of metallothionein in bronchoalveolar cells and lavage fluid following repeated cadmium inhalationEnvironmental Research, 1986
- Cellular and biochemical response of the rat lung to repeated inhalation of cadmiumToxicology and Applied Pharmacology, 1986
- Cellular localization of metallothionein in the lung following repeated cadmium inhalationToxicology, 1985
- Ability of Rabbit Alveolar Macrophages to Dissolve MetalsExperimental Lung Research, 1984
- Cd2+ responses of cultured human blood cellsToxicology and Applied Pharmacology, 1983
- Rapid solubilization and translocation of 109CdO following pulmonary depositionToxicology and Applied Pharmacology, 1980
- Early damage indicators in the lung. III. Biochemical and cytological response of the lung to inhaled metal saltsToxicology and Applied Pharmacology, 1979
- Isolation of a soluble cadmium-binding protein from pulmonary macrophagesToxicology and Applied Pharmacology, 1978