A chromatic explosion: the development and future of multiparameter flow cytometry
- 5 November 2008
- journal article
- review article
- Published by Wiley in Immunology
- Vol. 125 (4), 441-449
- https://doi.org/10.1111/j.1365-2567.2008.02989.x
Abstract
Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.Keywords
This publication has 77 references indexed in Scilit:
- Techniques to improve the direct ex vivo detection of low frequency antigen‐specific CD8+ T cells with peptide‐major histocompatibility complex class I tetramersCytometry Part A, 2008
- Gating‐ML: XML‐based gating descriptions in flow cytometryCytometry Part A, 2008
- MIFlowCyt: The minimum information about a flow cytometry experimentCytometry Part A, 2008
- Element-tagged immunoassay with ICP-MS detection: Evaluation and comparison to conventional immunoassaysJournal of Immunological Methods, 2008
- Polychromatic plots: Graphical display of multidimensional dataCytometry Part A, 2008
- Enhanced red and near infrared detection in flow cytometry using avalanche photodiodesCytometry Part A, 2008
- Statistical mixture modeling for cell subtype identification in flow cytometryCytometry Part A, 2008
- Polyfunctional T cell responses are a hallmark of HIV‐2 infectionEuropean Journal of Immunology, 2008
- Optimizing a Multicolor Immunophenotyping AssayClinics In Laboratory Medicine, 2007
- High-Content Flow Cytometry and Temporal Data Analysis for Defining a Cellular Signature of Graft-Versus-Host DiseaseTransplantation and Cellular Therapy, 2007