A high frequency of Mamu‐A *01 in the rhesus macaque detected by polymerase chain reaction with sequence‐specific primers and direct sequencing
- 11 December 1997
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 50 (6), 657-661
- https://doi.org/10.1111/j.1399-0039.1997.tb02927.x
Abstract
SIV infection of rhesus macaques is an excellent model for HIV infection of humans. Unfortunately, it is has been difficult to identify macaques expressing particular MHC class I alleles. Here we describe the use of PCR‐SSP for Mamu‐A *01 typing of rhesus macaques. The Mamu‐A *01 allele was amplified from genomic DNA using Mamu‐A *07‐specific primers and positive PCR products were directly sequenced. Our technique identified 15 Mamu‐A*01‐positive animals of 68 tested. We validated our molecular analysis by showing that lymphocytes from 8 Mamu‐A *01‐positive animals expressed Mamu‐A*01 as determined by immunoprecipitation and 1‐D IEF. The technical simplicity and accuracy of this typing method should facilitate selection of Mamu‐A *01‐positive rhesus macaques for AIDS virus pathogenesis and vaccine studies.Keywords
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