Root canal sealers cause cytotoxicity and oxidative damage in hepatocytes
- 1 January 2000
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 54 (3), 390-395
- https://doi.org/10.1002/1097-4636(20010305)54:3<390::aid-jbm110>3.0.co;2-u
Abstract
Elutable substances and degradation products from root canal sealers may gain access to periodontal tissue in many ways. The aim of this study was to investigate damage and oxidative stress caused by treatment with root canal sealers in primary cultured rat hepatocytes. Four root canal sealers were used: Canals, Sealapex, AH 26, and AH Plus. Toxicity was detected by assessing lactate dehydrogenase (LDH) leakage, and oxidative stress was determined by analysis of glutathione (GSH) levels. One‐way analysis of variance was performed on the data. As the concentrations of sealer were increased, LDH leakage increased (p < 0.01). Increased GSH depletion accompanied LDH leakage increase. GSH levels were reduced to zero at a concentration of 0.05% for Canals and 0.10% for AH 26. These results confirmed the hypothesis that root canal sealers can cause cytotoxicity in hepatocytes. The Canals and AH 26 sealers can also cause oxidative stress in hepatocytes. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 390–395, 2001Keywords
This publication has 22 references indexed in Scilit:
- Vitamin E protection of cell morphology under oxidative stress is related to cytoskeletal proteins in rat hepatocytesArchives of Toxicology, 1997
- In vitro evaluation of the cytotoxicity of calcium hydroxide‐based root canal sealersDental Traumatology, 1995
- Endodontic cements induce alterations in the cell cycle of in vitro cultured osteoblastsOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 1995
- S-Thiolation and Irreversible Oxidation of Sulfhydryls on Carbonic Anhydrase III During Oxidative Stress: A Method for Studying Protein Modification in Intact Cells and TissuesArchives of Biochemistry and Biophysics, 1994
- Tissue reaction to endodontic materials: Methods, criteria, assessment, and observationsOral Surgery, Oral Medicine, Oral Pathology, 1991
- Cytotoxicity of eugenol in sealer containing zinc‐oxideDental Traumatology, 1991
- Glutathionyl(cysteine-374) actin forms filaments of low mechanical stabilityBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1990
- Periodontally derived pulpal lesionsDental Traumatology, 1988
- ROLE OF REVERSIBLE OXIDATION-REDUCTION OF ENZYME THIOLS-DISULFIDES IN METABOLIC REGULATIONAnnual Review of Biochemistry, 1985
- High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfidesAnalytical Biochemistry, 1980