Population frequency, mutation linkage and analytical methodology for the Arg16Gly, Gln27Glu and Thr164Ile polymorphisms in the β 2 -adrenergic receptor among Turks
- 1 November 1999
- journal article
- clinical trial
- Published by Wiley in British Journal of Clinical Pharmacology
- Vol. 48 (5), 761-764
- https://doi.org/10.1046/j.1365-2125.1999.00082.x
Abstract
Aims Inherited polymorphisms of codons 16, 27, and 164 of the β2-adrenergic receptor (B2AR) gene may result in significantly changed functions of this receptor. The aim of the present study was to investigate the frequencies of the main mutations of the B2AR gene in Turks. Methods A group of 104 unrelated Turkish subjects were analysed for the Arg16Gly, Gln27Glu, and Thr164Leu polymorphisms of B2AR by a newly designed polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay. A 242-bp amplicon including the Arg16Gly and Gln27Glu polymorphisms was generated including an arbitrary restriction site, allowing the cleavage with Eco130I or Fnu4HI for analysis of the two polymorphic sites, respectively. For the amino acid polymorphism at codon 164 a 280-bp fragment was generated and digested by MnlI. Results The allelic frequencies of mutated Gly16, Glu27, and Ile164 alleles were found as 59.6%, 31.7%, and 0.5%, respectively. Although, the frequencies of the polymorphisms at codon 16 and 164 were found similar as in various Caucasian populations, the frequency of the Glu27 variant was found significantly lower than these populations. Conclusions Obviously there are interethnic differences in functionally relevant B2AR variants which may have implications for drug treatment and disease susceptibility.Keywords
This publication has 12 references indexed in Scilit:
- Low frequency of defective alleles of cytochrome P450 enzymes 2C19 and 2D6 in the Turkish populationClinical Pharmacology & Therapeutics, 1999
- Association between genetic polymorphisms of the beta2-adrenoceptor and response to albuterol in children with and without a history of wheezing.JCI Insight, 1997
- Association between β2-adrenoceptor polymorphism and susceptibility to bronchodilator desensitisation in moderately severe stable asthmaticsThe Lancet, 1997
- Polymorphisms of the β2-Adrenergic Receptor and AsthmaAmerican Journal of Respiratory and Critical Care Medicine, 1997
- The glutamine 27 β2-adrenoceptor polymorphism is associated with elevated IgE levels in asthmatic familiesJournal of Allergy and Clinical Immunology, 1997
- Genetic polymorphisms of the beta 2-adrenergic receptor in nocturnal and nonnocturnal asthma. Evidence that Gly16 correlates with the nocturnal phenotype.JCI Insight, 1995
- Beta 2 adrenergic receptor gene restriction fragment length polymorphism and bronchial asthma.Thorax, 1995
- Amino-Terminal Polymorphisms of the Human .beta.2-Adrenergic Receptor Impart Distinct Agonist-Promoted Regulatory PropertiesBiochemistry, 1994
- Mutations in the Gene Encoding for the β2-adrenergic Receptor in Normal and Asthmatic SubjectsAmerican Journal of Respiratory Cell and Molecular Biology, 1993
- cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.Proceedings of the National Academy of Sciences of the United States of America, 1987