Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii
- 8 August 2006
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 113 (6), 1049-1062
- https://doi.org/10.1007/s00122-006-0364-5
Abstract
Hexaploid wheat (Triticum aestivum L.) originated about 8,000 years ago from the hybridization of tetraploid wheat with diploid Aegilops tauschii Coss. containing the D-genome. Thus, the bread wheat D-genome is evolutionary young and shows a low degree of polymorphism in the bread wheat gene pool. To increase marker density around the durable leaf rust resistance gene Lr34 located on chromosome 7DS, we used molecular information from the orthologous region in rice. Wheat expressed sequence tags (wESTs) were identified by homology with the rice genes in the interval of interest, but were monomorphic in the ‘Arina’ × ‘Forno’ mapping population. To derive new polymorphic markers, bacterial artificial chromosome (BAC) clones representing a total physical size of ∼1 Mb and belonging to four contigs were isolated from Ae. tauschii by hybridization screening with wheat ESTs. Several BAC clones were low-pass sequenced, resulting in a total of ∼560 kb of sequence. Ten microsatellite sequences were found, and three of them were polymorphic in our population and were genetically mapped close to Lr34. Comparative analysis of marker order revealed a large inversion between the rice genome and the wheat D-genome. The SWM10 microsatellite is closely linked to Lr34 and has the same allele in the three independent sources of Lr34: ‘Frontana’, ‘Chinese Spring’, and ‘Forno’, as well in most of the genotypes containing Lr34. Therefore, SWM10 is a highly useful marker to assist selection for Lr34 in breeding programs worldwide.Keywords
This publication has 50 references indexed in Scilit:
- Development and use of anchored-SSRs to study DNA polymorphism in bread wheat (Triticum aestivum L.)Molecular Ecology Notes, 2006
- A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.)Theoretical and Applied Genetics, 2004
- In silico comparative analysis reveals a mosaic conservation of genes within a novel colinear region in wheat chromosome 1AS and rice chromosome 5SFunctional & Integrative Genomics, 2004
- Construction and characterization of a bacterial artificial chromosome (BAC) library of hexaploid wheat (Triticum aestivumL.) and validation of genome coverage using locus-specific primersGenome, 2003
- Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]Plant Cell, 2003
- Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markersTheoretical and Applied Genetics, 2002
- Simple inheritance of partial resistance to leaf rust in two wheat cultivarsPlant Pathology, 2001
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- The Deletion Stocks of Common WheatJournal of Heredity, 1996
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987