Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds
- 9 November 2011
- journal article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 48 (4), 522-524
- https://doi.org/10.1039/c1cc16039g
Abstract
A combination of classical site-directed mutagenesis, genetic code engineering and bioorthogonal reactions delivered a chemically modified barstar protein with one or four carbohydrates installed at specific residues. These protein conjugates were employed in multivalent binding studies, which support the use of proteins as structurally defined scaffolds for the presentation of multivalent ligands.Keywords
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