Human μ opiate receptor
Open Access
- 31 January 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 338 (2), 217-222
- https://doi.org/10.1016/0014-5793(94)80368-4
Abstract
A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium‐ and GTP‐sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24–25. An MspI polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.Keywords
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