High affinity RGD‐binding sites at the plasma membrane ofArabidopsis thalianalinks the cell wall
Open Access
- 1 October 1998
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 16 (1), 63-71
- https://doi.org/10.1046/j.1365-313x.1998.00276.x
Abstract
The heptapeptide Tyr‐Gly‐ Arg‐Gly‐Asp‐ Ser‐Pro containing the sequence Arg‐Gly‐Asp (RGD – the essential structure recognised by animal cells in substrate adhesion molecules) was tested on epidermal cells of onion and cultured cells of Arabidopsis upon plasmolysis. Dramatic changes were observed on both types of cells following treatment: on onion cells, Hechtian strands linking the cell wall to the membrane were lost, while Arabidopsis cells changed from concave to convex plasmolysis. A control heptapeptide Tyr‐Gly‐Asp‐Gly‐Arg‐Ser‐Pro had no effect on the shape of plasmolysed cells. Protoplasts isolated from Arabidopsis cells agglutinate in the presence of ProNectinF, a genetically engineered protein of 72 kDa containing 13 RGD sequences: several protoplasts may adhere to a single molecule of ProNectinF. The addition of the RGD‐heptapeptide disrupted the adhesion between the protoplasts. Purified plasma membrane from Arabidopsis cells exhibits specific binding sites for the iodinated RGD‐heptapeptide. The binding is saturable, reversible, and two types of high affinity sites (Kd1 1 nM, and Kd2 40 nM) can be discerned. Competitive inhibition by several structurally related peptides and proteins noted the specific requirement for the RGD sequence. Thus, the RGD‐binding activity of Arabidopsis fulfils the adhesion features of integrins, i.e. peptide specificity, subcellular location, and involvement in plasma membrane‐cell wall attachments.Keywords
This publication has 37 references indexed in Scilit:
- Inhibition of thigmostimulated cell differentiation with RGD-peptides inUromyces germlingsProtoplasma, 1996
- Localized membrane-wall adhesions inPelvetia zygotesProtoplasma, 1996
- Integrins: Emerging Paradigms of Signal TransductionAnnual Review of Cell and Developmental Biology, 1995
- Comparing plant and animal extracellular matrix-cytoskeleton connections ? are they alike?Protoplasma, 1995
- Integrin-ligarid interactions: a year in reviewCurrent Opinion in Cell Biology, 1994
- Mechanosensory calcium‐selective cation channels in epidermal cellsThe Plant Journal, 1993
- A Homolog of the Substrate Adhesion Molecule Vitronectin Occurs in Four Species of Flowering PlantsPlant Cell, 1991
- Solid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acidsInternational Journal of Peptide and Protein Research, 1990
- Rise in chlorotetracycline fluorescence accompanies tracheary element differentiation in suspension cultures ofZinniaProtoplasma, 1989
- Characterization of recombinant glycosylated human interleukin 2 produced by a recombinant plasmid transformed CHO cell lineFEBS Letters, 1987