CELL MEMBRANE DNA: A NEW TARGET FOR PSORALEN PHOTOADDUCT FORMATION
- 1 August 1990
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 52 (2), 315-321
- https://doi.org/10.1111/j.1751-1097.1990.tb04187.x
Abstract
The effects of 8‐methoxypsoralen plus long wavelength ultraviolet radiation on cell membrane DNA were examined. Treatment of human lymphocytes with 100 ng/ml 8‐methoxypsoralen and 5 J/cm2 UVA led to the formation of 7.1 ±3.8 photoadducts per million bases. A monoclonal antibody, specific for 8‐methoxypsoralen 4′,5′4nonoadducts, was used to detect photoadducts in the cell membrane DNA of human lymphocytes and three lymphoblastoid cell lines. Treatment of lymphocytes with 8‐MOP and UVA reduced the lymphocyte DNA binding capacity by 56%. Cell membranes of normal lymphocytes were shown to contain three high affinity DNA binding proteins of 28, 59, and 79 kDa, respectively.This publication has 19 references indexed in Scilit:
- Characterization of oligonucleotide transport into living cells.Proceedings of the National Academy of Sciences, 1989
- Inhibition of Antiskin Allograft Immunity by Infusions With Syngeneic Photoinactivated Effector Lymphocytes.Journal of Investigative Dermatology, 1989
- 6,4,4′-Trimethylangelicin photoadduct formation in DNA: Production and characterization of a specific monoclonal antibodyJournal of Photochemistry and Photobiology B: Biology, 1989
- Neoplastic Characteristics of the DNA Found in the Plasma of Cancer PatientsOncology, 1989
- Treatment of Cutaneous T-Cell Lymphoma by Extracorporeal PhotochemotherapyNew England Journal of Medicine, 1987
- EXTENSIVE CROSSLINKING BETWEEN SUBUNITS OF OLIGOMERIC PROTEINS INDUCED BY FUROCOUMARINS PLUS UV‐A IRRADIATIONPhotochemistry and Photobiology, 1986
- DNA binding to human leukocytes. Evidence for a receptor-mediated association, internalization, and degradation of DNA.JCI Insight, 1985
- A simple and rapid method for the preparation of plasma membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1983
- Penetration and localization of furocoumarins in single living cells studied by microspectrofluorometryBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1982
- Photochemotherapy of Psoriasis with Oral Methoxsalen and Longwave Ultraviolet LightNew England Journal of Medicine, 1974