Developmental changes in myocardial B cells mirror changes in B cells associated with different organs
Open Access
- 20 August 2020
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 5 (16)
- https://doi.org/10.1172/jci.insight.139377
Abstract
The naive heart harbors a population of intravascular B cells that make close contact with the cardiac microvasculature. However, the timing of their appearance and their organ specificity remain unknown. To address this knowledge gap, we performed a systematic analysis of B cells isolated from the myocardium and other organs, from embryonic life to adulthood. We found that the phenotype of myocardial B cells changed dynamically during development. While neonatal heart B cells were mostly CD11b+ and CD11b– CD21–CD23–, adult B cells were predominantly CD11b–CD21+CD23+. Histological analysis and intravital microscopy of lung and liver showed that organ-associated B cells in contact with the microvascular endothelium were not specific to the heart. Flow cytometric analysis of perfused hearts, livers, lungs, and spleen showed that the dynamic changes in B cell subpopulations observed in the heart during development mirrored changes observed in the other organs. Single cell RNA sequencing (scRNAseq) analysis of B cells showed that myocardial B cells were part of a larger population of organ-associated B cells that had a distinct transcriptional profile. These findings broaden our understanding of the biology of myocardial-associated B cells and suggest that current models of the dynamics of naive B cells during development are incomplete.Keywords
Funding Information
- NIH (R01 HL-58081,HL-73017-0,HL-089543-01,HL 111094,R00 HL138163,T32 HL007081,S10OD020136,1K08HL145108-01A1)
- Center for Cardiovascular Research at Washington University School of Medicine (Institutional Funds)
- McDonnell Genome Institute at Washington University (Pilot Grant)
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