Histopathological Effects and Growth Reduction in a Susceptible and a Resistant Strain of Heliothis virescens (Lepidoptera: Noctuidae) Caused by Sublethal Doses of Pure Cry1A Crystal Proteins from Bacillus thuringiensis

Abstract

Two strains of the tobacco budworm Heliothis virescens, one selected in the laboratory for resistance to Cry1Ac crystal protein from Bacillus thuringiensis (for which the mechanism of resistance was not associated with reduced binding) and an unselected control strain, were exposed to sublethal doses of pure Cry1A crystal proteins. A histopathological study was conducted to determine the epithelial damage caused by ingestion of Cry1Ac. Tissue sections of the midgut were obtained after 20, 40 and 60 min of toxin ingestion. Histopathological changes were observed primarily in columnar cells and were time-dependent. However, essentially the same level of damage was observed in the two strains. Toxin feeding tests with Cry1Ac and Cry1Ab, indicated that the toxins retarded growth and inhibited food intake of susceptible larvae, but did not significantly affect larvae of the resistant strain. Since the histopathological damage was similar in both strains, it appears that resistant larvae could repair (or substitute) more readily the damaged cells.