Nitric oxide induced by hydrogen peroxide mediates abscisic acid‐induced activation of the mitogen‐activated protein kinase cascade involved in antioxidant defense in maize leaves
- 18 April 2007
- journal article
- Published by Wiley in New Phytologist
- Vol. 175 (1), 36-50
- https://doi.org/10.1111/j.1469-8137.2007.02071.x
Abstract
* The role of nitric oxide (NO) and the relationship between NO, hydrogen peroxide (H(2)O(2)) and mitogen-activated protein kinase (MAPK) in abscisic acid (ABA)-induced antioxidant defense in leaves of maize (Zea mays) plants were investigated. * Both ABA and H(2)O(2) induced increases in the generation of NO in mesophyll cells of maize leaves, and H(2)O(2) was required for the ABA-induced generation of NO. Pretreatment with NO scavenger and nitric oxide synthase (NOS) inhibitor substantially reduced the ABA-induced production of NO, and partly blocked the activation of a 46 kDa MAPK and the expression and the activities of several antioxidant enzymes induced by ABA. Treatment with the NO donor sodium nitroprusside (SNP) also induced the activation of the MAPK, and enhanced the antioxidant defense systems. * Conversely, SNP treatment did not induce the production of H(2)O(2), and pretreatments with NO scavenger and NOS inhibitor did not affect ABA-induced H(2)O(2) production. * Our results suggest that ABA-induced H(2)O(2) production mediates NO generation, which, in turn, activates MAPK and results in the upregulation in the expression and the activities of antioxidant enzymes in ABA signaling.Keywords
This publication has 56 references indexed in Scilit:
- Mitogen-Activated Protein Kinase Is Involved in Abscisic Acid-Induced Antioxidant Defense and Acts Downstream of Reactive Oxygen Species Production in Leaves of Maize PlantsPlant Physiology, 2006
- ABA‐induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesisThe Plant Journal, 2005
- Emerging MAP kinase pathways in plant stress signallingTrends in Plant Science, 2005
- Cellular and Subcellular Localization of Endogenous Nitric Oxide in Young and Senescent Pea PlantsPlant Physiology, 2004
- Genetic Elucidation of Nitric Oxide Signaling in Incompatible Plant-Pathogen InteractionsPlant Physiology, 2004
- Nitric oxide: a new player in plant signalling and defence responsesCurrent Opinion in Plant Biology, 2004
- Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat‐induced photooxidative stress and to nitric oxide‐induced cell deathThe Plant Journal, 2004
- SALT AND DROUGHT STRESS SIGNAL TRANSDUCTION IN PLANTSAnnual Review of Plant Biology, 2002
- Role of Abscisic Acid in Water Stress-induced Antioxidant Defense in Leaves of Maize SeedlingsFree Radical Research, 2002
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976