Identification of a Nosema bombycis (Microsporidia) spore wall protein corresponding to spore phagocytosis
- 15 July 2011
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 138 (9), 1102-1109
- https://doi.org/10.1017/s0031182011000801
Abstract
SUMMARY: Life-cycle stages of the microsporidia Nosema bombycis, the pathogen causing silkworm pebrine, were separated and purified by an improved method of Percoll-gradient centrifugation. Soluble protein fractions of late sporoblasts (spore precursor cells) and mature spores were analysed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Protein spots were recovered from gels and analysed by mass spectrometry. The most abundant differential protein spot was identified by database search to be a hypothetical spore wall protein. Using immunoelectron microscopy, we demonstrated that HSWP5 is localized to the exospore of mature spores and renamed it as spore wall protein 5 (NbSWP5). Further spore phagocytosis assays indicated that NbSWP5 can protect spores from phagocytic uptake by cultured insect cells. This spore wall protein may function both for structural integrity and in modulating host cell invasion.Keywords
This publication has 22 references indexed in Scilit:
- Interaction of pathogenic yeasts with phagocytes: survival, persistence and escapeCurrent Opinion in Microbiology, 2010
- SWP25, A Novel Protein Associated with the Nosema bombycis Endospore1The Journal of Eukaryotic Microbiology, 2009
- Proteomic analysis of spore wall proteins and identification of two spore wall proteins from Nosema bombycis (Microsporidia)Proteomics, 2008
- EnP1, a Microsporidian Spore Wall Protein That Enables Spores To Adhere to and Infect Host Cells In VitroEukaryotic Cell, 2007
- EnP1 and EnP2, two proteins associated with the Encephalitozoon cuniculi endospore, the chitin-rich inner layer of the microsporidian spore wallInternational Journal for Parasitology, 2006
- Congruent evidence from α-tubulin and β-tubulin gene phylogenies for a zygomycete origin of microsporidiaFungal Genetics and Biology, 2003
- Developmental Expression of Two Spore Wall Proteins during Maturation of the Microsporidian Encephalitozoon intestinalisInfection and Immunity, 2001
- Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculiNature, 2001
- Fractionation of Different Life Cycle Stages of Microsporidia Nosema grylli from Crickets Gryllus bimaculatus by Centrifugation in Percoll Density Gradient for Biochemical ResearchThe Journal of Eukaryotic Microbiology, 1995
- Taxonomy of Phylum MicrosporaCritical Reviews in Microbiology, 1992