Activation of the Bactericidal Capacity of Polymorphonuclear Granulocytes after Surgery, Measured with a New in vitro Assay
- 1 July 1979
- journal article
- Published by Wiley in Scandinavian Journal of Haematology
- Vol. 23 (1), 10-16
- https://doi.org/10.1111/j.1600-0609.1979.tb02846.x
Abstract
A classic in vitro polymorphonuclear (PMN) granulocyte bactericidal system was used alongside a newly developed modification to see whether the new assay would increase the possibility to detect a stimulation of PMN bactericidal functions. In the new assay each granulocyte was provided with 30--40 bacteria, which is quite close to the maximal killing capacity (usually 60 bacteria per PMN). Granulocytes were obtained from 8 patients the day before, the day after and 2 d after they underwent thoracotomy with cardiopulmonary by-pass (CPS). The granulocytes from all patients showed an increased capacity to kill Staph. aureus in vitro 2 d after the operation, compared to before, when the submaximal bacterial concentration per granulocyte was used, whereas no change was observed with the standard bacterial concentration (3--4 bacteria per granulocyte). Thus, the new assay might make it possible to observe an enhanced PMN bactericidal ability.Keywords
This publication has 10 references indexed in Scilit:
- Fasting (Acute Energy Deprivation) in Man: Effect on Polymorphonuclear Granulocyte Functions, Plasma Iron and Serum TransferrinScandinavian Journal of Haematology, 2009
- Obesity, Plasma Lipids and Polymorphonuclear (PMN) Granulocyte FunctionsScandinavian Journal of Haematology, 2009
- Polymorphonuclear (PMN) Function after Small Intestinal Shunt Operation for Morbid ObesityBritish Journal of Haematology, 1980
- Ageing Does Not Change Blood Granulocyte Bactericidal Capacity and Levels of Complement Factors 3 and 4Gerontology, 1978
- Stressor exposure and immunological response in man: Interferon-producing capacity and phagocytosisJournal of Psychosomatic Research, 1976
- Inhibition of Granulocyte Adherence by Ethanol, Prednisone, and Aspirin, Measured with an Assay SystemThe New England Journal of Medicine, 1974
- Transient defects in phagocytic functions during cardiopulmonary bypassThe Journal of Thoracic and Cardiovascular Surgery, 1974
- NEUTROPHIL GRANULOCYTE FUNCTION IN BACTERIAL INFECTIONSThe Lancet, 1972
- FATAL GRANULOMATOUS DISEASE OF CHILDHOOD: An Inborn Abnormality of Phagocytic FunctionThe Lancet, 1966