Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla
- 1 December 2015
- journal article
- Published by Wiley in International Endodontic Journal
- Vol. 49 (12), 1132-1140
- https://doi.org/10.1111/iej.12571
Abstract
To evaluate the effects of two types of calcium silicate cements on viability, angiogenic growth factor release, and angiogenic and inflammation-related gene expression in human stem cells from the apical papilla (SCAP). SCAPs were grown for 7 days with either ProRoot mineral trioxide aggregate (MTA) or Biodentine (BD). Cell viability and media concentrations of vascular endothelial growth factor (VEGF/VEGFA) and angiopoietin 1 (ANGPT1) were measured. The expression of genes related to angiogenic potential and inflammatory response was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). One-way and two-way analyses of variance with multiple comparisons Tukey's test were performed (P < 0.05). Cells in contact with either cement were associated with increased cell viability compared with the no-treatment group at day 1 but there were no differences amongst groups at days 3 and 7. Exposure to either cement significantly increased VEGF concentrations at day 3; however, ANGPT-1 levels decreased significantly compared with the no-treatment group at day 3. Exposure to MTA and BD stimulated expression of VEGFA and FIGF/VEGFD. Furthermore, exposure to both cements significantly decreased the mRNA levels of ANGPT1 and FGF2 relative to the no-treatment group. Both MTA and BD stimulated the expression of angiogenic genes and release of VEGF, inducing similar expression patterns; however, they appeared to inhibit the expression of specific genes, including ANGPT1 and FGF2.Keywords
Funding Information
- Pilot Project Research Funds, University of the Pacific, Arthur A. Dugonic School of Dentistry (097)
This publication has 28 references indexed in Scilit:
- Role of the P38 Pathway in Calcium Silicate Cement–induced Cell Viability and Angiogenesis-related Proteins of Human Dental Pulp Cell In VitroJournal of Endodontics, 2014
- Effects of 3 Endodontic Bioactive Cements on Osteogenic Differentiation in Mesenchymal Stem CellsJournal of Endodontics, 2014
- Role of the p38 pathway in mineral trioxide aggregate‐induced cell viability and angiogenesis‐related proteins of dental pulp cell in vitroInternational Endodontic Journal, 2014
- Regeneration of the dentine–pulp complex with revitalization/revascularization therapy: challenges and hopesInternational Endodontic Journal, 2013
- Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA AngelusDental Materials, 2013
- Investigation of the physical properties of tricalcium silicate cement-based root-end filling materialsDental Materials, 2013
- Direct Contact with Mineral Trioxide Aggregate Activates and Differentiates Human Dental Pulp CellsJournal of Endodontics, 2011
- Induction of specific cell responses to a Ca3SiO5-based posterior restorative materialDental Materials, 2008
- N-acetylcysteine protects dental pulp stromal cells from HEMA-induced apoptosis by inducing differentiation of the cellsFree Radical Biology & Medicine, 2007
- Increased Vascularization in Mice Overexpressing Angiopoietin-1Science, 1998