Designing of a Si-MEMS device with an integrated skeletal muscle cell-based bio-actuator
- 19 October 2010
- journal article
- Published by Springer Science and Business Media LLC in Biomedical Microdevices
- Vol. 13 (1), 123-129
- https://doi.org/10.1007/s10544-010-9477-3
Abstract
With the aim of designing a mechanical drug delivery system involving a bio-actuator, we fabricated a Micro Electro Mechanical Systems (MEMS) device that can be driven through contraction of skeletal muscle cells. The device is composed of a Si-MEMS with springs and ratchets, UV-crosslinked collagen film for cell attachment, and C2C12 muscle cells. The Si-MEMS device is 600 μm × 1000 μm in size and the width of the collagen film is 250 ~ 350 μm, which may allow the device to go through small blood vessels. To position the collagen film on the MEMS device, a thermo-sensitive polymer was used as the sacrifice-layer which was selectively removed with O2 plasma at the positions where the collagen film was glued. The C2C12 myoblasts were seeded on the collagen film, where they proliferated and formed myotubes after induction of differentiation. When C2C12 myotubes were stimulated with electric pulses, contraction of the collagen film-C2C12 myotube complex was observed. When the edge of the Si-MEMS device was observed, displacement of ~8 μm was observed, demonstrating the possibility of locomotive movement when the device is placed on a track of adequate width. Here, we propose that the C2C12-collagen film complex is a new generation actuator for MEMS devices that utilize glucose as fuel, which will be useful in environments in which glucose is abundant such as inside a blood vessel.Keywords
This publication has 28 references indexed in Scilit:
- Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum‐free medium AIM‐VBiotechnology & Bioengineering, 2009
- Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capabilityBiotechnology & Bioengineering, 2009
- Plasma surface modification of poly(D,L‐lactic acid) as a tool to enhance protein adsorption and the attachment of different cell typesJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2008
- A modular instrument for exploring the mechanics of cardiac myocytesAmerican Journal of Physiology-Heart and Circulatory Physiology, 2007
- Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubesExperimental Cell Research, 2007
- Force-length relations in isolated intact cardiomyocytes subjected to dynamic changes in mechanical loadAmerican Journal of Physiology-Heart and Circulatory Physiology, 2007
- Myotube Assembly on Nanofibrous and Micropatterned PolymersNano Letters, 2006
- Controlling the Direction of Kinesin-Driven Microtubule Movements along Microlithographic TracksBiophysical Journal, 2001
- Excitability and contractility of skeletal muscle engineered from primary cultures and cell linesAmerican Journal of Physiology-Cell Physiology, 2001
- A MEMS electrostatic particle transportation systemSensors and Actuators A: Physical, 1999