Gene therapy used for tissue engineering applications
- 1 March 2007
- journal article
- review article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 59 (3), 329-350
- https://doi.org/10.1211/jpp.59.3.0002
Abstract
This review highlights the advances at the interface between tissue engineering and gene therapy. There are a large number of reports on gene therapy in tissue engineering, and these cover a huge range of different engineered tissues, different vectors, scaffolds and methodology. The review considers separately in-vitro and in-vivo gene transfer methods. The in-vivo gene transfer method is described first, using either viral or non-viral vectors to repair various tissues with and without the use of scaffolds. The use of a scaffold can overcome some of the challenges associated with delivery by direct injection. The ex-vivo method is described in the second half of the review. Attempts have been made to use this therapy for bone, cartilage, wound, urothelial, nerve tissue regeneration and for treating diabetes using viral or non-viral vectors. Again porous polymers can be used as scaffolds for cell transplantation. There are as yet few comparisons between these many different variables to show which is the best for any particular application. With few exceptions, all of the results were positive in showing some gene expression and some consequent effect on tissue growth and remodelling. Some of the principal advantages and disadvantages of various methods are discussed.Keywords
This publication has 100 references indexed in Scilit:
- Impregnation of Plasmid DNA into Three-Dimensional Scaffolds and Medium Perfusion Enhancein VitroDNA Expression of Mesenchymal Stem CellsTissue Engineering, 2005
- Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regenerationMolecular Therapy, 2005
- Lentivirus-mediated gene transfer induces long-term transgene expression of BMP-2 in vitro and new bone formation in vivoMolecular Therapy, 2005
- Combination of porous hydroxyapatite and cationic liposomes as a vector for BMP-2 gene therapyBiomaterials, 2004
- Bone induction by BMP‐2 transduced stem cells derived from human fatJournal of Orthopaedic Research, 2003
- Inhibition of cartilage degradation: A combined tissue engineering and gene therapy approachArthritis & Rheumatism, 2003
- Sustained Effects of Gene-Activated Matrices after CNS InjuryMolecular and Cellular Neuroscience, 2001
- Osteoinduction by Bone Morphogenetic Protein-2 via Adenoviral Vector under Transient ImmunosuppressionBiochemical and Biophysical Research Communications, 2000
- A Novel Gene Delivery System Using Urothelial Tissue Engineered Neo-OrgansJournal of Urology, 1997
- Enhanced function of cultured epithelium by genetic modification: Cell-based synthesis and delivery of growth factorsBiotechnology & Bioengineering, 1996