Fibroblast Growth Factor Receptor-1 (FGFR1) Nuclear Dynamics Reveal a Novel Mechanism in Transcription Control
- 1 May 2009
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 20 (9), 2401-2412
- https://doi.org/10.1091/mbc.e08-06-0600
Abstract
Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nuclear matrix. Factors (cAMP, CBP) that induce FGFR1-mediated gene activation shifted FGFR1 from the nuclear matrix (immobile) to chromatin (slow) and reduced the movement rate of the chromatin-bound population. Transcription inhibitors accelerated FGFR1 movement; the content of the chromatin-bound slow FGFR1 decreased, whereas the fast population increased. The transcriptional activation appears to involve conversion of the immobile matrix-bound and the fast nuclear FGFR1 into a slow chromatin-binding population through FGFR1's interaction with CBP, RSK1, and the high-molecular-weight form of FGF-2. Our findings support a general mechanism in which gene activation is governed by protein movement and collisions with other proteins and nuclear structures.Keywords
This publication has 44 references indexed in Scilit:
- Direct Regulation of rRNA Transcription by Fibroblast Growth Factor 2Molecular and Cellular Biology, 2005
- 90-kDa Ribosomal S6 Kinase Is a Direct Target for the Nuclear Fibroblast Growth Factor Receptor 1 (FGFR1)Published by Elsevier BV ,2004
- Rapid Glucocorticoid Receptor Exchange at a Promoter Is Coupled to Transcription and Regulated by Chaperones and ProteasomesMolecular and Cellular Biology, 2004
- Nuclear trafficking of FGFR1: A role for the transmembrane domainJournal of Cellular Biochemistry, 2003
- Differential Intranuclear Localization of Fibroblast Growth Factor-2 Isoforms and Specific Interaction with the Survival of Motoneuron ProteinJournal of Biological Chemistry, 2003
- The transcription cycle of RNA polymerase II in living cellsThe Journal of cell biology, 2002
- Importin β–Mediated Nuclear Import of Fibroblast Growth Factor ReceptorThe Journal of cell biology, 2001
- Fluorescence resonance energy transfer microscopy: a mini reviewJournal of Biomedical Optics, 2001
- The Glucocorticoid Receptor: Rapid Exchange with Regulatory Sites in Living CellsScience, 2000
- Core filaments of the nuclear matrix.The Journal of cell biology, 1990