Circulation of Two Mumps Virus Genotypes in an Unimmunized Population in India
- 8 May 2013
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 85 (8), 1426-1432
- https://doi.org/10.1002/jmv.23600
Abstract
Two separate outbreaks of fever with parotitis were reported from the Apsinga and Pimpla villages in the Osmanabad district of the Maharashtra State, India during February and March 2012. Meningo-encephalitis was noted in two patients resulting in the death of an 11-year male. Samples of blood and throat swabs were collected from patients with fever and parotitis. Serum samples from suspected (n = 62) and convalescent (n = 19) patients were tested for mumps virus specific IgM and/or IgG antibodies. Mumps virus specific IgM antibodies were detected in 44 of 62 serum samples (71%). Of the 19 convalescent phase sera 16 had both, anti-mumps virus IgM and IgG antibodies. Twenty-eight throat swabs collected from patients with parotitis were tested by RT-PCR for the SH gene. Twenty-three specimens were found to be positive and nucleotide sequencing of the amplified PCR products revealed circulation of two distinct genotypes that were village specific. Mumps virus genotype C (n = 18) was detected in Apsinga village and genotype G (n = 5) in Pimpla village. Two mumps virus isolates were also obtained using Vero cells. This is the first report from India confirming simultaneous circulation of mumps virus genotype C in one village and the G genotype in another village only 37 km away. J. Med. Virol. 85:1426–1432, 2013.Keywords
This publication has 17 references indexed in Scilit:
- MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony MethodsMolecular Biology and Evolution, 2011
- Mumps outbreak in a highly vaccinated student population, The Netherlands, 2004Vaccine, 2010
- Role of laboratory in rapid diagnosis of atypical mumpsThe Brazilian Journal of Infectious Diseases, 2010
- Comparisons of mumps virus potency estimates obtained by 50% cell culture infective dose assay and plaque assayVaccine, 2010
- Development of a focus reduction neutralization test (FRNT) for detection of mumps virus neutralizing antibodiesJournal of Virological Methods, 2010
- MumpsThe Lancet, 2008
- Proposal for genetic characterisation of wild-type mumps strains: Preliminary standardisation of the nomenclatureArchiv für die gesamte Virusforschung, 2005
- Genetic Heterogeneity of Mumps Virus in the United Kingdom: Identification of Two New GenotypesThe Journal of Infectious Diseases, 1999
- Virus and host cell-dependent variation in transcription of the mumps virus genomeJournal of General Virology, 1990
- Molecular Cloning and Characterization of Six Genes, Determination of Gene Order and Intergenic Sequences and Leader Sequence of Mumps VirusJournal of General Virology, 1988