Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency
- 1 July 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (7), 2612-2614
- https://doi.org/10.1128/jcm.00449-06
Abstract
We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.Keywords
This publication has 19 references indexed in Scilit:
- Selection and persistence of non-nucleoside reverse transcriptase inhibitor-resistant HIV-1 in patients starting and stopping non-nucleoside therapyAIDS, 2006
- Comparative performance evaluation of the HIV-1 LiPA protease and reverse transcriptase resistance assay on clinical isolatesJournal of Clinical Virology, 2005
- Sensitive Phenotypic Detection of Minor Drug-Resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase VariantsJournal of Clinical Microbiology, 2005
- Multiple, Linked Human Immunodeficiency Virus Type 1 Drug Resistance Mutations in Treatment-Experienced Patients Are Missed by Standard Genotype AnalysisJournal of Clinical Microbiology, 2005
- Emergence of Minor Populations of Human Immunodeficiency Virus Type 1 Carrying the M184V and L90M Mutations in Subjects Undergoing Structured Treatment InterruptionsThe Journal of Infectious Diseases, 2003
- Evolution of Human Immunodeficiency Virus Type 1 Populations after Resumption of Therapy following Treatment Interruption and Shift in Resistance GenotypeThe Journal of Infectious Diseases, 2002
- Underestimation of HIV Type 1 Drug Resistance Mutations: Results from the ENVA-2 Genotyping Proficiency ProgramAIDS Research and Human Retroviruses, 2002
- Replication of Phenotypically Mixed Human Immunodeficiency Virus Type 1 Virions Containing Catalytically Active and Catalytically Inactive Reverse TranscriptaseJournal of Virology, 2001
- Changes in Human Immunodeficiency Virus Type 1 Populations after Treatment Interruption in Patients Failing Antiretroviral TherapyJournal of Virology, 2001
- Monitoring Resistance to Human Immunodeficiency Virus Type 1 Protease Inhibitors by PyrosequencingJournal of Clinical Microbiology, 2001