Overexpression of interleukin-12 and T helper 1 predominance in lupus nephritis
- 15 September 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 154 (2), 247-254
- https://doi.org/10.1111/j.1365-2249.2008.03758.x
Abstract
Summary: Imbalance of cytokine homeostasis is a prominent feature of both experimental and human systemic lupus erythematosus (SLE). Because interleukin (IL)-12 promotes interferon (IFN)-γ production leading to polarization of peripheral cells toward a T helper (Th) 1 phenotype, we investigated its role in lupus nephritis (LN). Soluble Th1 and Th2 cytokines were measured by enzyme-linked immunosorbent assay (ELISA) in sera and urines of SLE patients and controls. Th1/Th2 peripheral lymphocyte polarization was determined by flow cytometry. Glomerular accumulation of IL-12 was evaluated by immunohistochemistry, whereas urinary IL-12 was evaluated by ELISA. Higher serum IL-12 levels in SLE were associated with LN, whereas IL-4 was unrelated to the renal damage. Peripheral cells from LN patients showed a Th1 phenotype with a high IFN-γ expression that paralleled the severity of renal damage. IL-12 was present within glomerular mononuclear cells in classes IV and V LN, and its accumulation was correlated strongly with urinary levels. IL-12 overexpression in SLE may contribute to the development of LN. Both serum and urinary IL-12 elevation reflect its glomerular production and parallel Th1 polarization of peripheral T cells and high IFN-γ production. In SLE patients, IL-12 measurement may thus be predictive of the development of LN.Keywords
This publication has 45 references indexed in Scilit:
- IL-23 drives a pathogenic T cell population that induces autoimmune inflammationThe Journal of Experimental Medicine, 2005
- New approaches to the renal pathogenicity of anti-DNA antibodies in systemic lupus erythematosusAutoimmunity Reviews, 2004
- IMPAIRED PRODUCTION OF IL-12 IN SYSTEMIC LUPUS ERYTHEMATOSUS. III: DEFICIENT IL-12p40 GENE EXPRESSION AND CROSS-REGULATION OF IL-12, IL-10 AND IFN-γ GENE EXPRESSIONCytokine, 1999
- IMPAIRED PRODUCTION OF IL-12 IN SYSTEM LUPUS ERYTHEMATOSUS. II: IL-12 PRODUCTION IN VITRO IS CORRELATED NEGATIVELY WITH SERUM IL-10, POSITIVELY WITH SERUM IFN-γ AND NEGATIVELY WITH DISEASE ACTIVITY IN SLECytokine, 1998
- Decreased Th1-like and Increased Th2-like Cells in Systemic Lupus ErythematosusScandinavian Journal of Rheumatology, 1998
- Updating the American college of rheumatology revised criteria for the classification of systemic lupus erythematosusArthritis & Rheumatism, 1997
- Glomerular ICAM-1 Expression Related to Circulating TNF-α in Human GlomerulonephritisNephron, 1997
- Disease severity in patients with systemic lupus erythematosus correlates with an increased ratio of interleukin‐10: Interferon‐γ–secreting cells in the peripheral bloodArthritis & Rheumatism, 1996
- Immune response-associated production of neopterin. Release from macrophages primarily under control of interferon-gamma.The Journal of Experimental Medicine, 1984
- RENAL INVOLVEMENT IN SYSTEMIC LUPUS ERYTHEMATOSUS (SLE)Medicine, 1978