Environment Control to Improve Recombinant Protein Yields in Plants Based on Agrobacterium-Mediated Transient Gene Expression
Open Access
- 8 March 2016
- journal article
- review article
- Published by Frontiers Media SA in Frontiers in Bioengineering and Biotechnology
- Vol. 4, 23
- https://doi.org/10.3389/fbioe.2016.00023
Abstract
Agrobacterium-mediated transient expression systems enable plants to produce a wide range of recombinant proteins on a rapid timescale. To achieve economically feasible upstream production and downstream processing, two yield parameters should be considered: 1) recombinant protein content per unit biomass; and 2) recombinant protein productivity per unit area-time at the end of the upstream production. Because environmental factors in the upstream production have impacts on those parameters, environment control is important to maximize the recombinant protein yield. In this review, we summarize the effects of pre- and post-inoculation environmental factors in the upstream production on the yield parameters and discuss the basic concept of environment control for plant-based transient expression systems. Pre-inoculation environmental factors associated with planting density, light quality and nutrient supply affect plant characteristics such as biomass and morphology, which in turn affect recombinant protein content and productivity. Accordingly, environment control for such plant characteristics has significant implications to achieve a high yield. On the other hand, post-inoculation environmental factors such as temperature, light intensity and humidity have been shown to affect recombinant protein content. Considering that recombinant protein production in Agrobacterium-mediated transient expression systems is a result of a series of complex biological events starting from T-DNA transfer from Agrobacterium tumefaciens to protein biosynthesis and accumulation in leaf tissue, we propose that dynamic environment control during the post-inoculation process, i.e., changing environmental conditions at an appropriate timing for each event, may be a promising approach to obtain a high yield. Detailed descriptions of plant growth conditions and careful examination of environmental effects will significantly contribute to our knowledge to stably obtain high recombinant protein content and productivity, thus enhancing the utility of plant-based transient expression systems as recombinant protein factories.Keywords
Funding Information
- Japan Society for the Promotion of Science (KAKENHI 26712021)
- Ministry of Agriculture, Forestry and Fisheries (Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry 25025A)
This publication has 42 references indexed in Scilit:
- Bipartite and tripartite Cucumber mosaic virus-based vectors for producing the Acidothermus cellulolyticus endo-1,4-β-glucanase and other proteins in non-transgenic plantsBMC Biotechnology, 2012
- Recovery and purification of plant-made recombinant proteinsBiotechnology Advances, 2012
- GMP issues for recombinant plant-derived pharmaceutical proteinsBiotechnology Advances, 2012
- Biomass allocation to leaves, stems and roots: meta‐analyses of interspecific variation and environmental controlNew Phytologist, 2011
- Emerging antibody products and Nicotiana manufacturingHuman Vaccines, 2011
- HIV-1 Neutralization Profile and Plant-Based Recombinant Expression of Actinohivin, an Env Glycan-Specific Lectin Devoid of T-Cell Mitogenic ActivityPLOS ONE, 2010
- The production of hemagglutinin‐based virus‐like particles in plants: a rapid, efficient and safe response to pandemic influenzaPlant Biotechnology Journal, 2010
- Plant-derived hemagglutinin protects ferrets against challenge infection with the A/Indonesia/05/05 strain of avian influenzaVaccine, 2009
- Plant-produced idiotype vaccines for the treatment of non-Hodgkin's lymphoma: Safety and immunogenicity in a phase I clinical studyProceedings of the National Academy of Sciences of the United States of America, 2008
- Transfer and Integration of T-DNA without Cell Injury in the Host PlantPlant Cell, 1997