Capillary Electrophoresis Coupled with Electrochemiluminescence Detection Using Porous Etched Joint
- 4 May 2004
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 76 (13), 3846-3850
- https://doi.org/10.1021/ac049743j
Abstract
A new setup to couple capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection is described in which the electrical connection of CE is achieved through a porous section at a distance of 7 mm from the CE capillary outlet. Because the porous capillary wall allowed the CE current to pass through and there was no electric field gradient beyond that section, the influence of CE high-voltage field on the ECL procedure was eliminated. The porous section formed by etching the capillary with hydrofluoric acid after only one side of the circumference of 2−3 mm of polyimide coating of the capillary was removed, while keeping the polyimide coating on the other part to protect the capillary from HF etching makes the capillary joint much more robust since only a part of the circumference of it is etched. A standard three-electrode configuration was used in experiments with Pt wire as a counter electrode, Ag/AgCl as a reference electrode, and a 300-μm diameter Pt disk as a working electrode. Compared with CE-ECL conventional decoupler designs, the present setup with a porous joint has no added dead volume created. Moreover, the dead volume can be increasingly decreased by shortening the distance (∼100 μm) between the working electrode and the end of the separation capillary. The versatility in choice of capillaries and separation buffers within this design is the main advantage over the use of small i.d. capillary and low conductivity buffer in some CE-ECL systems. The performance of this setup is illustrated by the analyses of tripropylamine and proline.Keywords
This publication has 15 references indexed in Scilit:
- Capillary Electrophoresis to Mass Spectrometry Interface Using a Porous JunctionAnalytical Chemistry, 2003
- Recent applications of electrogenerated chemiluminescence in chemical analysisTalanta, 2001
- Electrochemically generated Ru(bpy)33+-based chemiluminescence detection in micellar electrokinetic chromatographyTalanta, 2000
- Compositional analysis of small peptides using capillary electrophoresis and Ru(bpy)33+-based chemiluminescence detectionMicrochemical Journal, 2000
- In situ cell for electrochemically generated Ru(bpy)33+-based chemiluminescence detection in capillary electrophoresisAnalytica Chimica Acta, 1999
- A review of recent trends in analytical applications of electrogenerated chemiluminescenceTrAC Trends in Analytical Chemistry, 1999
- Tris (2,2′‐bipyridyl)ruthenium (III) as a chemiluminescent reagent for detection in capillary electrophresisJournal of High Resolution Chromatography, 1997
- On-line electrogenerated Ru(bpy)33+ chemiluminescent detection of β-blockers separated with capillary electrophoresisAnalytica Chimica Acta, 1997
- Validation of a modified spectrophotometric method for the determination of nitrate in dry milk using 2-sec-butylphenolThe Analyst, 1995
- Selective Determination of Oxalate with a Ruthenium(II) Complex/Nafion-Modified Electrode Combined with a Carbon Dioxide SensorAnalytical Sciences, 1994