Translation attenuation regulation of chloramphenicol resistance in bacteria — a review
- 31 December 1996
- journal article
- review article
- Published by Elsevier BV in Gene
- Vol. 179 (1), 157-162
- https://doi.org/10.1016/s0378-1119(96)00420-9
Abstract
The chloramphenicol (Cm)-inducible cat and cmlA genes are regulated by translation attenuation, a regulatory device that modulates mRNA translation. In this form of gene regulation, translation of the CmR coding sequence is prevented by mRNA secondary structure that sequesters its ribosome-binding site (RBS). A translated leader of nine codons precedes the secondary structure, and induction results when a ribosome becomes stalled at a specific site in the leader. Here we demonstrate that the site of ribosome stalling in the leader is selected by a cis effect of the nascent leader peptide on its translating ribosome.Keywords
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