The muscarinic receptor subtype in mouse pancreatic B‐cells
- 15 August 1988
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 236 (1), 89-92
- https://doi.org/10.1016/0014-5793(88)80290-4
Abstract
Isolated mouse islets were used to identify the muscarinic receptor subtype present in pancreatic B-cells. We thus compared the inhibitory potencies of atropine (non-specific), of pirenzepine (specific for M1 receptors) and of compound AF-DX 116 (specific for cardiac M2 receptors) on acetylcholine-induced insulin release, 86Rb+ efflux and 45Ca2+ efflux. The three antagonists inhibited all effects of acetylcholine, but EC50 values were markedly different: atropine = 1.5–5 nM, pirenzepine = 0.6–1.7 μM and AF-DX 116 = 1.7–11 μM. The results did not suggest that the various effects of ACh could result from the activation of different subtypes of receptors. It is concluded that muscarinic receptors of pancreatic B-cells belong to an M2 subtype distinct from the cardiac M2 receptorsKeywords
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