Blood volatile compounds as biomarkers for colorectal cancer
Open Access
- 1 November 2013
- journal article
- Published by Informa UK Limited in Cancer Biology & Therapy
- Vol. 15 (2), 200-206
- https://doi.org/10.4161/cbt.26723
Abstract
Many recent studies have focused on the connection between the composition of specific volatile organic compounds (VOCs) in exhaled breath and various forms of cancer. However, the composition of exhaled breath is affected by many factors, such as lung disease, smoking, and diet. VOCs are released into the bloodstream before they are exhaled; therefore, the analysis of VOCs in blood will provide more accurate results than the analysis of VOCs in exhaled breath. Blood were collected from 16 colorectal cancer patients and 20 healthy controls, then solid phase microextraction–chromatography–mass spectrometry (SPME-GC-MS) was used to analysis the exhaled volatile organic compounds (VOCs). The statistical methods principal component analysis (PCA) and partial least-squares discriminant analysis (PLSDA) were performed to deal with the final dates. Three metabolic biomarkers were found at significantly lower levels in the group of CRC patients than in the normal control group (P < 0.01): phenyl methylcarbamate, ethylhexanol, and 6-t-butyl-2,2,9,9-tetramethyl-3,5-decadien-7-yne. In addition, significantly higher levels of 1,1,4,4-tetramethyl-2,5-dimethylene-cyclohexane were found in the group of CRC patients than in the normal control group (P < 0.05). Compared with healthy individuals, patients with colorectal adenocarcinoma exhibited a distinct blood metabolic profile with respect to VOCs. The analysis of blood VOCs appears to have potential clinical applications for CRC screening.Keywords
This publication has 43 references indexed in Scilit:
- Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRBGastroenterology Research and Practice, 2012
- A Novel Serum Metabolomics-Based Diagnostic Approach for Colorectal CancerPLOS ONE, 2012
- Cancer treatment and survivorship statistics, 2012CA: A Cancer Journal for Clinicians, 2012
- Prediction of gastric cancer metastasis through urinary metabolomic investigation using GC/MSWorld Journal of Gastroenterology, 2011
- Detection of lung, breast, colorectal, and prostate cancers from exhaled breath using a single array of nanosensorsBritish Journal of Cancer, 2010
- α-Hydroxybutyrate Is an Early Biomarker of Insulin Resistance and Glucose Intolerance in a Nondiabetic PopulationPLOS ONE, 2010
- Release of volatile organic compounds (VOCs) from the lung cancer cell line CALU-1 in vitroCancer Cell International, 2008
- Diagnostic potential of breath analysis—focus on volatile organic compoundsClinica Chimica Acta; International Journal of Clinical Chemistry, 2004
- Diet and cancer — the European Prospective Investigation into Cancer and NutritionNature Reviews Cancer, 2004
- Randomised study of screening for colorectal cancer with faecal-occult-blood testThe Lancet, 1996