Cellulase inhibition by tannins in the testa of field beans (Vicia faba)
- 31 October 1977
- journal article
- research article
- Published by Wiley in Journal of the Science of Food and Agriculture
- Vol. 28 (11), 983-989
- https://doi.org/10.1002/jsfa.2740281106
Abstract
The testa of field bean varieties with coloured flowers contained 4–8 % tannin (measured as total phenolics) compared with less than 0.6 % in the testa of white flowered varieties. The tannin present in whole beans was almost entirely contained in the testa. The presence of high levels of tannin reduced in vitro digestibility and solubility in cellulase solutions of the testa and addition of high tannin testa decreased the solubility of cotyledon protein in acid pepsin. Water extracts of the testa from coloured‐flower varieties inhibited the action of fungal cellulase and reduced the in vitro digestibility of white flowered testa and cellulose. A highly significant relationship (r= −0.93***) was found between the tannin content and solubility in cellulase of testa for a range of 24 varieties. It is concluded that the effect of testa tannins on in vitro digestibility can be mainly attributed to their effect on cell wall digesting enzymes rather than an effect on protein solubility per se.This publication has 9 references indexed in Scilit:
- In vitro digestibility of the testa in tannin-free field beans (Vicia faba L.)The Journal of Agricultural Science, 1976
- A comparison of the protein nutritive value and composition of four cultivars of faba beans (vicia faba l.) grown and harvested under controlled conditionsJournal of the Science of Food and Agriculture, 1975
- The effect of pepsin pretreatment of herbage on the prediction of dry matter digestibility from solubility in fungal cellulase solutionsJournal of the Science of Food and Agriculture, 1975
- Effect of Methionine and Choline on Tannic Acid and Tannin Toxicity in the Laying HenPoultry Science, 1973
- Noncentral ForcesAmerican Journal of Physics, 1966
- THE TANNINSPublished by Elsevier BV ,1965
- BURNSPlastic and Reconstructive Surgery, 1963
- A TWO‐STAGE TECHNIQUE FOR THE IN VITRO DIGESTION OF FORAGE CROPSGrass and Forage Science, 1963
- Leuco-anthocyanins. 2. Systematic distribution of leuco-anthocyanins in leavesBiochemical Journal, 1954