The use of ion exchangers in the preparation and purification of cytochrome c

Abstract

A simple and reliable procedure using cation exchangers to prepare 0.43% iron cytochrome c from the 0.34% Fe prepn. from horse heart is descr. The product is as active enzymically (per [mu] mole of cytochrome c iron) as the prepn. containing 0.34% Fe. This technique is used for the prepn. of cytochrome c in either the reduced or oxidized state, in a stable form. The non-cytochrome fraction of 0.34% Fe cytochrome c prepns. is identified as globin from myoglobin. Under suitable conditions cytochrome c can react with myoglobin or the globin prepared from it.