Platelets secrete stromal cell–derived factor 1α and recruit bone marrow–derived progenitor cells to arterial thrombi in vivo
Open Access
- 17 April 2006
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 203 (5), 1221-1233
- https://doi.org/10.1084/jem.20051772
Abstract
The accumulation of smooth muscle and endothelial cells is essential for remodeling and repair of injured blood vessel walls. Bone marrow–derived progenitor cells have been implicated in vascular repair and remodeling; however, the mechanisms underlying their recruitment to the site of injury remain elusive. Here, using real-time in vivo fluorescence microscopy, we show that platelets provide the critical signal that recruits CD34+ bone marrow cells and c-Kit+ Sca-1+ Lin− bone marrow–derived progenitor cells to sites of vascular injury. Correspondingly, specific inhibition of platelet adhesion virtually abrogated the accumulation of both CD34+ and c-Kit+ Sca-1+ Lin− bone marrow–derived progenitor cells at sites of endothelial disruption. Binding of bone marrow cells to platelets involves both P-selectin and GPIIb integrin on platelets. Unexpectedly, we found that activated platelets secrete the chemokine SDF-1α, thereby supporting further primary adhesion and migration of progenitor cells. These findings establish the platelet as a major player in the initiation of vascular remodeling, a process of fundamental importance for vascular repair and pathological remodeling after vascular injury.Keywords
This publication has 45 references indexed in Scilit:
- Interactions of Platelets with Subendothelium and EndotheliumMicrocirculation, 2005
- Rapamycin attenuates vascular wall inflammation and progenitor cell promoters after angioplastyThe FASEB Journal, 2004
- Generation of Functional Culture–Derived Platelets From CD34 + Progenitor Cells to Study Transgenes in the Platelet EnvironmentCirculation Research, 2004
- Soluble glycoprotein VI dimer inhibits platelet adhesion and aggregation to the injured vessel wall in vivoThe FASEB Journal, 2003
- Origin of Smooth Muscle Progenitor Cells: Different Conclusions From Different ModelsCirculation, 2003
- Fluvastatin Enhances the Inhibitory Effects of a Selective Angiotensin II Type 1 Receptor Blocker, Valsartan, on Vascular Neointimal FormationCirculation, 2003
- A Critical Role of Platelet Adhesion in the Initiation of Atherosclerotic Lesion FormationThe Journal of Experimental Medicine, 2002
- Adhesion of Activated Platelets to Endothelial Cells: Evidence for a GPIIbIIIa-dependent Bridging Mechanism and Novel Roles for Endothelial Intercellular Adhesion Molecule 1 (ICAM-1), αvβ3 Integrin, and GPIbαThe Journal of Experimental Medicine, 1998
- Platelet-Mediated Lymphocyte Delivery to High Endothelial VenulesScience, 1996
- Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem CellScience, 1996