Activity of superoxide dismutase isoenzymes in lens epithelial cells derived from different types of age-related cataract
- 1 March 2008
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Cataract & Refractive Surgery
- Vol. 34 (3), 470-474
- https://doi.org/10.1016/j.jcrs.2007.10.044
Abstract
To evaluate the level of superoxide dismutase (SOD) and SOD isoenzyme activity in lens epithelial cells (LECs) derived from different types of cataract in patients having phacoemulsification. Iladevi Cataract & IOL Research Centre, Memnagar, Ahmedabad, India. This observational study of 109 patients having phacoemulsification was performed to evaluate the level of activity in LECs of total superoxide dismutase (TSOD) and 2 superoxide dismutase isoenzymes: copper- (Cu) and zinc (Zn)-dependent SOD (Cu/Zn-SOD) and manganese (Mn)-dependent SOD (Mn-SOD). The curvilinear capsulorhexis (lens capsule) harboring LECs was obtained during phacoemulsification. The anterior lens capsule samples were processed for assaying SOD activity using the nitro blue tetrazolium reduction assay. The samples were grouped based on age and on pure cataract types. The highest level of TSOD, Cu/Zn-SOD, and Mn-SOD activity was in patients 50 years or younger. The activity declined gradually with age (P<.001). The level of TSOD activity increased in cortical cataract. The level of Cu/Zn-SOD and Mn-SOD isoenzyme activity in LECs was higher in cortical cataracts. A significant difference in the level of TSOD and Cu/Zn-SOD activity was found with different types of cataract (P<.001). The level of TSOD, Cu/Zn-SOD, and Mn SOD isoenzyme activity decreased with age in LECs of patients with age-related cataract. A significant difference in the level of TSOD and Cu/Zn-SOD isoenzyme activity between different types of cataract was observed. The activity of all 3 SOD isoenzymes was highest in cortical cataracts.Keywords
This publication has 20 references indexed in Scilit:
- Oxygen, Reactive Oxygen Species and Tissue DamageCurrent Pharmaceutical Design, 2004
- Associations of cataract with antioxidant enzymes and other risk factors: The French Age-Related Eye Diseases (POLA) Prospective StudyOphthalmology, 2003
- Lens superoxide dismutase and catalase activities in diabetic cataractClinical Biochemistry, 2002
- Blood and lens lipid peroxidation and antioxidant status in normal individuals, senile and diabetic cataractous patientsCurrent Eye Research, 2002
- Free radicals, exercise and antioxidant supplementationProceedings of the Nutrition Society, 1998
- Blood lipid peroxidation products and antioxidants in senile cataractIndian Journal of Clinical Biochemistry, 1996
- Stop, chop, chop, and stuffJournal of Cataract & Refractive Surgery, 1996
- An introduction to free radical biochemistryBritish Medical Bulletin, 1993
- Lipid peroxidation in cataract of the humanLife Sciences, 1986
- Superoxide Dismutase Molecules in Human Cataractous LensesOphthalmic Research, 1986