ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro
Open Access
- 1 January 2013
- journal article
- research article
- Published by Hindawi Limited in Mediators of Inflammation
- Vol. 2013, 1-16
- https://doi.org/10.1155/2013/216402
Abstract
Microglia are the immune cells in the central nervous system. After injury microglia release bioactive molecules, including cytokines and ATP, which modify the functional state of hemichannels (HCs) and gap junction channels (GJCs), affecting the intercellular communication via extracellular and intracellular compartments, respectively. Here, we studied the role of extracellular ATP and several cytokines as modulators of the functional state of microglial HCs and GJCs using dye uptake and dye coupling techniques, respectively. In microglia and the microglia cell line EOC20, ATP advanced the TNF-/IFN--induced dye coupling, probably through the induction of IL-1 release. Moreover, TNF-/IFN-, but not TNF- plus ATP, increased dye uptake in EOC20 cells. Blockade of Cx43 and Panx1 HCs prevented dye coupling induced by TNF-/IFN-, but not TNF- plus ATP. In addition, IL-6 prevented the induction of dye coupling and HC activity induced by TNF-/IFN-Keywords
Funding Information
- Consejo Nacional de Ciencia y Tecnología (24100062, 1111033, 1090353, DO7I1086, AQ-1507)
This publication has 80 references indexed in Scilit:
- ATP and glutamate released via astroglial connexin 43 hemichannels mediate neuronal death through activation of pannexin 1 hemichannelsJournal of Neurochemistry, 2011
- FGF-1 induces ATP release from spinal astrocytes in culture and opens pannexin and connexin hemichannelsProceedings of the National Academy of Sciences of the United States of America, 2010
- Connexon‐mediated cell adhesion drives microtissue self‐assemblyThe FASEB Journal, 2010
- Differentially altered Ca2+ regulation and Ca2+ permeability in Cx26 hemichannels formed by the A40V and G45E mutations that cause keratitis ichthyosis deafness syndromeThe Journal of general physiology, 2010
- ATP Released by Electrical Stimuli Elicits Calcium Transients and Gene Expression in Skeletal MusclePublished by Elsevier BV ,2009
- IFN-γ receptor signaling mediates spinal microglia activation driving neuropathic painProceedings of the National Academy of Sciences of the United States of America, 2009
- Modulation of Brain Hemichannels and Gap Junction Channels by Pro-Inflammatory Agents and Their Possible Role in NeurodegenerationAntioxidants and Redox Signaling, 2009
- Connexin Hemichannel Composition Determines the FGF-1–induced Membrane Permeability and Free [Ca2+]iResponsesMolecular Biology of the Cell, 2008
- Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X7 receptorThe EMBO Journal, 2006
- Functional implications of calcium permeability of the channel formed by pannexin 1The Journal of cell biology, 2006